Respiratory Enzymes Used for Perchlorate Reduction

0001900洛根：这项研究的目的是阐明高氯酸盐还原微生物(PRM)的生理学。在地下水中检测到高氯酸盐，它危及1200多万人的饮用水供应。高氯酸盐可被许多新分离的细菌菌株用作电子受体。有证据表明，在高氯酸盐还原的最后一步，亚氯酸盐歧化酶产生分子氧。这是一个有趣的生物学发展，因为氧是首选的电子受体。在完全好氧的条件下，即使是有能力的细菌也不能还原高氯酸盐。硝酸盐也会干扰高氯酸盐的还原。使用呼吸抑制剂，研究人员将探测PRM中呼吸链上的电子转移，以确定高氯酸盐和氯酸盐如何用于细胞呼吸。通过使用其他化学物质，如氯霉素来抑制蛋白质合成，将确定不同的途径是可诱导的还是构成的，以及在细菌培养中保持高氯酸盐还原条件所必需的条件。这项研究将为发展饮用水、废水和现场处理系统以生物去除高氯酸盐提供必要的信息。

0001900 Logan The objective of this research is to elucidate the physiology of perchlorate reducing microorganisms (PRMs). Perchlorate has been detected in ground waters and it endangers the drinking water supply of more than twelve million people. Perchlorate can be used as an electron acceptor by many newly isolated strains of bacteria. Evidence suggests that in the last step of perchlorate reduction, a chlorite dismutase produces molecular oxygen. This is an interesting biological development because oxygen is a preferred electron acceptor. Under fully aerobic conditions, even capable bacteria do not reduce perchlorate. Nitrates can also interfere with perchlorate reduction. Using respiratory inhibitors, the researchers will probe the transfer of electrons along the respiratory chain in PRMs in order to determine how perchlorate and chlorate are used for cell respiration. Through the use of other chemicals, such as chloramphenicol to inhibit protein synthesis, it will be determined whether different pathways are inducible or constitutive and what conditions are necessary to maintain perchlorate-reducing conditions in bacterial cultures. This research will provide information necessary for the development of drinking water, wastewater and in-situ treatment systems to biologically remove perchlorate.***