CAREER: Genetic, Molecular and Physiological Studies of Lateral Root Development in Arabidopsis thaliana

9722191威廉姆斯育种计划通常忽略了对根系的改造，因为它不容易获得。对于例如根结构或体系结构的改进，不可能进行直接选择。选拔方案是间接的。拟南芥是鉴定控制根构型基因的理想模型。为了鉴定侧根形成率异常的根，将对M2诱变种子进行筛选。只有那些初级根的生长速度和形态没有改变的植物才会被追寻。将对剩余的突变体进行广泛的遗传分析，包括与野生型的回交，以及构建双突变组合。选定的基因座将被克隆。此外，选择基因的保守区将用于侧根cDNA文库的扩增。将确定根特异基因的表达模式和编码基因产物的功能。拟议的工作将主要由本科生、暑期项目和所需的高级研究项目进行。该项目将更好地理解侧根形成的可塑性和参与侧根形成的基因。

9722191 Williams Breeding programs have typically neglected, due to its inaccessibility, modification of the root system. No direct selection is possible for the improvement of, e.g., root structure or architecture. Selection schemes are indirect. Arabidopsis is an ideal model in which to identify the genes controlling root architecture. To identify roots with abnormal rates of lateral root formation, M2 mutagenized seeds will be screened. Only those plants in which the growth rate and morphology of the primary root is not altered will be pursued. An extensive genetic analysis of the remaining mutants will be carried out, including backcrosses into the wild type, and construction of double mutant combinations. Selected loci will be cloned. In addition, conserved regions of select genes will be used for the amplification of a lateral root cDNA library. The expression pattern of the root-specific genes and the function of the encoded gene products will be ascertained. The proposed work will be conducted primarily by undergraduate students, in summer programs and in required senior research projects. A better understanding of the plasticity of lateral root formation and of genes involved in lateral root formation will result from this project.