ERA NanoSci - Molecular Machines that use structured DNA for directed movement

ERA NanoSci - 使用结构化 DNA 进行定向运动的分子机器

• 批准号: 118128451
• 负责人: Professor Dr. Ralf Seidel
• 金额: --
• 依托单位: Instituto de Engenharia de Sistemas e Computadores para os Microsistemas e Nanotecnologias
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2009
• 资助国家: 德国
• 起止时间: 2008-12-31 至 2013-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/118128451?language=en
• 关键词: ERA; NanoSci; Molecular; Machines; use

We propose to develop a dynamic nanotool for use in drug discovery. This nanotool is based around a nanoactuator that uses a biological molecular motor to manipulate DNA and attached objects, in this case a magnetic bead. An electronic sensing system, based on MagnetoResistive (MR) devices will detect motion of the magnetic bead and output an electronic signal. Both the DNA and the motors can be targets for drugs, which means the device can be used in screening drug-target interactions. The major advantage this system offers is single molecule detection and reporting of events, which will improve sensitivity to limits that are possible. The concept will be expanded by making novel use of branched and hairpin structures to provide information about position of binding of DNA-binding proteins, which will allow us to identify drugs targeted at non-motor DNA-binding proteins such as transcription factors.

我们建议开发一种用于药物发现的动态纳米工具。这种纳米工具是基于一种纳米致动器，该致动器使用生物分子马达来操纵DNA和附着的物体，在这种情况下是磁珠。基于磁阻(MR)器件的电子传感系统将检测磁珠的运动并输出电子信号。DNA和马达都可以成为药物的靶标，这意味着该设备可以用于筛选药物与靶标的相互作用。该系统提供的主要优势是单分子检测和事件报告，这将提高对可能的限制的敏感度。这一概念将通过使用分支和发夹结构的新用途来扩展，以提供有关DNA结合蛋白结合位置的信息，这将使我们能够识别针对非运动DNA结合蛋白的药物，如转录因子。