Cloning and Characterization of a New Locus RGA Involved in Gibberellin Signal Transduction in Arabidopsis

97-23171 Sun赤霉素是一个大家族的二萜类化合物，其中许多是植物中的生长调节剂，其影响不同的发育过程，如发芽、茎伸长、叶扩展或花和果实或种子发育。拟议的工作集中在赤霉素的细胞和信号转导途径，导致已知的激素反应，导致基因表达的变化和发育和生理反应，是很好地描述的感知。相反，人们对赤霉素的感知和信号传导知之甚少。本论文将对可能编码赤霉素受体的基因RGA进行表征，并研究拟南芥中赤霉素信号转导不同方面缺陷的突变体。 这项工作包括遗传分析、基因克隆和传统的基因特征鉴定技术、细胞特异性表达以及最后的基因功能描述生化分析。虽然这项工作主要是为了了解激素信号，但这项工作在作物改良等方面的应用是相当可观的。

97-23171 Sun Gibberellins are a large family of diterpenoid compounds, many of which are growth regulators in plants which affect diverse developmental processes, such as germination, stem elongation, leaf expansion, or flower and fruit or seed development. The proposed work focuses on the perception of gibberellins by cells and the signal transduction pathway that leads to the known responses of the hormone resulting in changes in gene expression and the developmental and physiological reactions that are well described. Little, in contrast, is known about gibberellin perception and signaling. The proposed will characterize the gene, RGA, which very likely encodes a gibberellin receptor, and study mutants in Arabidopsis which are defective in different aspects of gibberellin signal transduction. The work includes genetic analysis, gene cloning and the traditional techniques for characterizations of genes, cell-specificity of expression and, finally, biochemical analyses for a functional description of genes. While the work is principally designed for obtaining an understanding of hormone signaling, the application aspects of the work for crop improvement, for example, are considerable.