U.S.-Cooperative Science: Regulatory Mechanisms of Gibberellin Biosynthesis and response in Arabidopsis

小行星9603418 该奖项支持北卡罗来纳州杜克大学孙太平教授和日本琦玉理化研究所（RIKEN）的Yuji Kamiya博士之间为期三年的合作研究项目。 本计画是研究赤霉素在拟南芥中的生物合成与反应的调控机制。 赤霉素（GAs）是一类二萜类化合物，其中一些是植物生长调节剂，控制种子萌发、茎伸长和花发育等多种过程。 本项目主要有三个目标：1）研究光对拟南芥GA生物合成途径中第一个基因（GA 1）表达的影响，以了解光如何调节响应组织中GA浓度; 2）研究前两个酶过量产生的影响（对映贝壳杉烯合成A和B）;和3）测量已经分离的新GA响应突变体的GA含量以确定这些突变是否改变GA代谢。 该项目汇集了两个实验室的努力，这两个实验室构成了该过程的不同部分：美国人将提供新的GA反应突变体，日本人将进行GA测量。 该项目的结果将确定GA生物合成的调控机制和器官特异性GA反应，现在还不清楚。 通过交流思想和技术，该项目将扩大我们的基础知识，促进国际理解与合作。

9603418 Sun This award supports a three year collaborative research project between Professor Tai-ping Sun of Duke University in North Carolina and Dr. Yuji Kamiya of the Institute of Physical and Chemical Research (RIKEN) in Saitama, Japan. The project is a study of the regulatory mechanisms of gibberellin biosynthesis and response in Arabidopsis. Gibberellins (GAs) are diterpenoid compounds, some of which are plant growth regulators, controlling such diverse processes as seed germination, stem elongation, and flower development. There are three major goals of this project: 1) examine the effect of light on the expression of the first gene (GA1) in the Arabidopsis GA biosynthetic pathway to understand how light regulates GA concentrations in responding tissues; 2) examine the effects of overproduction of the first two enzymes (ent- kaurene syntheses A and B) in the GA biosynthetic pathway on plant growth; and 3) measure the GA content of new GA-response mutants that have been isolated to determine whether these mutations alter GA metabolism. The project brings together the efforts of two laboratories that have constituted different parts of the process: the Americans will provide the new GA-response mutants and the Japanese will perform the GA measurements. The results of this project will identify regulatory mechanisms of GA biosynthesis and organ-specific GA responses that are now unclear. Through the exchange of ideas and technology, this project will broaden our base of basic knowledge and promote international understanding and cooperation.