Molecular Mechanism of Gibberellin Signaling Repression by the DELLA Protein RGA in Arabidopsis

拟南芥DELLA蛋白RGA抑制赤霉素信号传导的分子机制

• 批准号: 0641548
• 负责人: Tai-ping Sun
• 金额: $ 65万
• 依托单位: Duke University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-04-15 至 2011-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0641548&HistoricalAwards=false
• 关键词: Molecular; Mechanism; Gibberellin; Signaling; Repression

IOS-0641548 (Tai-ping Sun)Title: Molecular Mechanism of Gibberellin Signaling Repression by the DELLA Protein RGA in ArabidopsisAbstract Gibberellin (GA) is an important plant growth hormone, which promotes seed germination, leaf expansion, stem elongation and flower development in higher plants. The dwarf cultivars of wheat and rice developed during the 'Green Revolution' in the 60s remarkably increased the grain yields. Recent studies revealed that these cultivars are modified in their GA production or responses to GA, illustrating the important role of this hormone in regulating plant development and in agriculture. GA-promoted growth and development is modulated by DELLA proteins, which are major negative regulators (repressors) of GA responses. GA, upon binding to its receptor, activates its response pathway by causing rapid degradation of DELLA. DELLA proteins are likely to function as transcriptional regulators and recent studies identified several key regulatory genes that are targets of DELLA in the model plant Arabidopsis. Two of these genes, one encodes transcription regulator (SCL3) and the other encodes an enzyme (RING) that may regulate stability of its downstream target protein(s). Genetic, molecular and biochemical approaches will be taken to investigate functions of SCL3 and RING in GA-regulated plant growth and development. DELLA proteins are functionally highly conserved in Arabidopsis and in many crop plants. For example, the semi-dwarf wheat cultivars, an essential component of the Green Revolution, contain mutations in the Rht loci that encode DELLA proteins. This study will likely to have a broader impact in improving quality of agricultural crops. In addition, a postdoctoral fellow and four undergraduate students will gain research experience in this project. The findings of this study will be incorporated into four undergraduate courses (including one lab course) at Duke University. Students will learn to appreciate how the information gained from studies in a model plant system can be used to improve agriculture practice and benefit society.

IOS-0641548（孙太平）题目：拟南芥中DELLA蛋白RGA抑制赤霉素信号传导的分子机制摘要赤霉素（GA）是一种重要的植物生长激素，在高等植物中促进种子萌发、叶片扩张、茎伸长和花发育。 六十年代“绿色革命”期间培育的小麦和水稻矮化品种显着提高了粮食产量。 最近的研究表明，这些品种的 GA 产量或对 GA 的反应发生了改变，说明了这种激素在调节植物发育和农业中的重要作用。 GA 促进的生长和发育由 DELLA 蛋白调节，DELLA 蛋白是 GA 反应的主要负调节因子（阻遏因子）。 GA 与其受体结合后，通过导致 DELLA 快速降解来激活其反应途径。 DELLA 蛋白可能起到转录调节因子的作用，最近的研究确定了模式植物拟南芥中 DELLA 的几个关键调节基因。 其中两个基因，一个编码转录调节因子 (SCL3)，另一个编码可调节其下游靶蛋白稳定性的酶 (RING)。 将采用遗传、分子和生物化学方法来研究SCL3和RING在GA调节的植物生长和发育中的功能。 DELLA 蛋白在拟南芥和许多农作物中功能高度保守。 例如，半矮化小麦品种（绿色革命的重要组成部分）在编码 DELLA 蛋白的 Rht 基因座中含有突变。 这项研究可能会对提高农作物的质量产生更广泛的影响。 此外，一名博士后研究员和四名本科生将在该项目中获得研究经验。 这项研究的结果将被纳入杜克大学的四门本科课程（包括一门实验课程）。 学生将学会如何利用从模型植物系统研究中获得的信息来改进农业实践并造福社会。