Regulation of Tryptophan Genes in Arabidopsis

9723172本工作将研究拟南芥中色氨酸的生物合成。这一途径的基因是由饥饿、伤害、病原体攻击诱导的，这导致氨基酸的产生增加，最终导致次生代谢物的产生，而次生代谢产物对调节发育或防止病原体的损害至关重要。这项工作的目标是表征控制色氨酸基因表达的分子因素。大多数实验将集中于对该途径中的第一个基因/酶--ASA1的调控。影响ASA1表达的突变体(ATR)已经被分离出来，将对其进行鉴定，以试图确定调控的遗传位点。其他实验将使用生化技术和转基因报告基因表达来研究转录和翻译控制。ATR1的特性将阐明该因子在连接环境信号和控制色氨酸基因表达方面的作用。

9723172 Bender The work will study tryptophan biosynthesis in Arabidopsis. Genes for this pathway are induced by starvation, wounding, pathogen attack and this results in the increased production of the amino acid and, eventually, secondary metabolites which are important to adjusting development or preventing damage by pathogens. The goal of the work is to characterize the molecular factors that control tryptophan gene expression. Most experiments will focus on regulation of the first gene/ enzyme in the pathway, ASA1. Mutants (ATR) that affect ASA1 expression have been isolated which will be characterized in an attempt to identify regulatory genetic loci. Other experiments will use biochemical techniques and transgenic reporter gene expression for the study of transcriptional and translational control. The characterization of ATR1 will elucidate the role of this factor in connecting environmental signals with control of tryptophan gene expression.