Regulation of Tryptophan Genes in Arabidopsis

拟南芥中色氨酸基因的调控

• 批准号: 9723172
• 负责人: Judith Bender
• 金额: $ 34.5万
• 依托单位: Johns Hopkins University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-01-01 至 2002-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9723172&HistoricalAwards=false
• 关键词: Regulation; Tryptophan; Genes; Arabidopsis

9723172 Bender The work will study tryptophan biosynthesis in Arabidopsis. Genes for this pathway are induced by starvation, wounding, pathogen attack and this results in the increased production of the amino acid and, eventually, secondary metabolites which are important to adjusting development or preventing damage by pathogens. The goal of the work is to characterize the molecular factors that control tryptophan gene expression. Most experiments will focus on regulation of the first gene/ enzyme in the pathway, ASA1. Mutants (ATR) that affect ASA1 expression have been isolated which will be characterized in an attempt to identify regulatory genetic loci. Other experiments will use biochemical techniques and transgenic reporter gene expression for the study of transcriptional and translational control. The characterization of ATR1 will elucidate the role of this factor in connecting environmental signals with control of tryptophan gene expression.

9723172 Bender这项工作将研究拟南芥中色氨酸的生物合成。这一途径的基因被饥饿、伤害、病原体攻击诱导，这导致氨基酸的产生增加，并最终导致次级代谢物的产生，这对调节发育或防止病原体的损害很重要。这项工作的目的是表征控制色氨酸基因表达的分子因素。大多数实验将集中于该通路中第一个基因/酶ASA1的调控。影响ASA1表达的突变体（ATR）已经被分离出来，将被表征，试图确定调控基因位点。其他实验将使用生化技术和转基因报告基因表达来研究转录和翻译控制。ATR1的特性将阐明该因子在连接环境信号与色氨酸基因表达控制中的作用。