Molecular Regulation of Isoprenoid Metabolism in Plant- Pathogen Interactions

9724126查佩尔植物类异戊二烯包括一组结构多样的化合物，可分为初级和次级代谢物两类。异戊二烯类化合物是植物的初级代谢产物，包括甾醇、类胡萝卜素和生长激素，次级代谢产物包括单萜、倍半萜和二萜，它们是植物-环境相互作用的重要介体。这项工作将继续对组织培养中的次生代谢产物进行生化/生理学研究。重点将放在该途径中两种酶的调节和活性上，角鲨烯合成酶和倍半萜环化酶位于生化途径分叉处，导致不同的化合物。分析还包括对这些酶的基因和转录本的分析。

9724126 Chappell Plant isoprenoids comprise a structurally diverse group of compounds that can be divided into classes of primary and secondary metabolites. Isoprenoids that are primary metabolites include sterols, carotenoids and growth hormones, secondary metabolites include monoterpenes, sesquiterpenes, and diterpenes, compounds that are important mediators of plant-environment interactions. The work will continue biochemical/physiological studies of the secondary metabolites in tissue culture. The focus will be on the regulation and activity of two enzymes in the pathway, squalene synthase and sesquiterpene cyclase which are located at branchpoints at which the biochemical pathways diverge leading to different compounds. The analysis also includes analysis of the genes and transcripts for these enzymes.