Molecular Regulation of Isoprenoid Metabolism in Plant- Pathogen Interactions

9724126 Chappell植物类异戊二烯包括一组结构多样的化合物，可分为初级代谢物和次级代谢物。类异戊二烯的初级代谢物包括固醇、类胡萝卜素和生长激素，次级代谢物包括单萜、倍半萜和二萜，这些化合物是植物与环境相互作用的重要介质。这项工作将继续进行组织培养中次生代谢物的生化/生理研究。重点将放在途径中的两种酶的调节和活性上，角鲨烯合成酶和倍半萜环化酶位于生化途径分化导致不同化合物的分支点上。分析还包括对这些酶的基因和转录本的分析。

9724126 Chappell Plant isoprenoids comprise a structurally diverse group of compounds that can be divided into classes of primary and secondary metabolites. Isoprenoids that are primary metabolites include sterols, carotenoids and growth hormones, secondary metabolites include monoterpenes, sesquiterpenes, and diterpenes, compounds that are important mediators of plant-environment interactions. The work will continue biochemical/physiological studies of the secondary metabolites in tissue culture. The focus will be on the regulation and activity of two enzymes in the pathway, squalene synthase and sesquiterpene cyclase which are located at branchpoints at which the biochemical pathways diverge leading to different compounds. The analysis also includes analysis of the genes and transcripts for these enzymes.