Molecular Regulation of Isoprenoid Metabolism in Plant-Pathogen Interactions

植物-病原体相互作用中类异戊二烯代谢的分子调控

• 批准号: 9729590
• 负责人: Joseph Chappell
• 金额: $ 30.47万
• 依托单位: University of Kentucky
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-06-15 至 2001-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9729590&HistoricalAwards=false
• 关键词: Molecular; Regulation; Isoprenoid; Metabolism; Plant

9729590 Chappell Plant isoprenoids comprise a structurally diverse group of compounds; some are essential for plant growth and development and others are important mediators of plant-environment interactions. For example, when attenuated pathogens are added to solanaceous plant cell cultures, the cultures cease sterol production (essential metabolite), and instead synthesize and secrete antibiotic sesquiterpenes. The decline in sterol biosynthesis has been correlated with the suppression of one key enzyme (squalene synthase), and the induction of sesquiterpene biosynthesis with the induction of another key enzyme (sesquiterpene cyclase). The PI's long range goal is to understand how this control is imposed onto these two key enzymes. The current objectives are to evaluate how two particular signal molecules might orchestrate this regulation, and to examine how several molecular mechanisms might contribute to the regulation of squalene synthase and sterol metabolism. These studies should provide important insights into how this pathway can be manipulated for enhanced value using genetic engineering technology.

9729590 Chappell Plant异戊二烯类化合物包括一组结构多样的化合物；一些是植物生长发育所必需的，另一些是植物与环境相互作用的重要媒介。例如，当将减毒病原体添加到茄属植物细胞培养物中，培养物停止产生固醇（必需代谢物），而是合成和分泌抗生素倍半萜。甾醇生物合成的下降与一种关键酶（角鲨烯合成酶）的抑制有关，而另一种关键酶（倍半萜环化酶）的诱导则与倍半萜的生物合成有关。PI的长期目标是了解这种控制是如何强加于这两种关键酶的。目前的目标是评估两种特定的信号分子如何协调这种调节，并检查几种分子机制如何有助于调节角鲨烯合成酶和固醇代谢。这些研究应该为如何利用基因工程技术操纵这一途径以提高价值提供重要的见解。