U.S.-Australia Cooperative Research: Regulation of the Ryanodine-Receptor

美国-澳大利亚合作研究：Ryanodine 受体的调节

• 批准号: 9724904
• 负责人: Esther Gallant
• 金额: $ 3.87万
• 依托单位: University of Minnesota-Twin Cities
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-01-15 至 1999-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9724904&HistoricalAwards=false
• 关键词: U.S.; Australia; Cooperative; Research; Regulation

9724904 Gallant Under this U.S.-Australia cooperative research award, Dr. Esther Gallant, Department of Veterinary Biology, University of Minnesota, and Dr. Angela F. Dulhunty, John Curtin School of Medical Research, Australian National University, Canberra, will join forces to study the regulation of the ryanodine receptor in skeletal muscle excitation- contraction. Skeletal muscle excitation-contraction coupling involves the interaction of two calcium channel proteins, the dihydropyridine receptor and the ryanodine-receptor. A short dihydropyradine peptide sequence is required for in vivo excitation-contraction coupling and can substitute for the dihydropyridine in ryanodine-receptor activation. The investigators will record currents through ryanodine- receptor channels inserted in artificial lipid bilayer membranes to determine 1) concentrations of coproteins and ligands necessary for optimal ryanodine-receptor function, and 2) ryanodine-receptor activation by dihydropyridine receptor peptides under these optimal conditions. The U.S. and Australian investigators are experts in the area of excitation-contraction coupling in muscle. Both will approach the study from different perspectives: Prof. Gallant has focused at the cellular and tissue level; while Prof. Dulhunty has experience with the single-channel, artificial bilayer technique. Prof. Gallant will employ the new knowledge gained in Australia to develop a similar apparatus in Minnesota. In addition, this project will provide an opportunity for Dr. Gallant's graduate student to participate in research in Australia.

9724904根据这项美澳合作研究奖，明尼苏达大学兽医生物学系的埃斯特·加兰特博士和堪培拉澳大利亚国立大学约翰·柯廷医学院的安吉拉·F·杜伦蒂博士将联手研究兰尼定受体在骨骼肌兴奋-收缩中的调节。骨骼肌兴奋收缩偶联涉及二氢吡啶受体和兰尼定受体两种钙通道蛋白的相互作用。体内兴奋-收缩偶联需要一个较短的二氢吡啶多肽序列，在兰诺定受体激活过程中可以替代二氢吡啶。研究人员将记录通过插入人工脂质双层膜的兰诺受体通道的电流，以确定1)最佳兰诺受体功能所需的辅蛋白和配体的浓度，以及2)二氢吡啶受体多肽在这些最佳条件下激活兰诺受体的情况。美国和澳大利亚的调查人员是肌肉兴奋-收缩耦合领域的专家。两人都将从不同的角度处理这项研究：加兰特教授专注于细胞和组织层面；而杜伦蒂教授则拥有单通道人工双层技术的经验。加兰特教授将利用在澳大利亚获得的新知识，在明尼苏达州开发一个类似的仪器。此外，该项目将为加兰特博士的研究生提供在澳大利亚参与研究的机会。