SGER: Efficient Extrachromosomal Replication of Exogenous DNA by a Filamentous Fungus

SGER：丝状真菌对外源 DNA 进行高效染色体外复制

• 批准号: 9724999
• 负责人: David Long
• 金额: $ 4.96万
• 依托单位: Montana State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-07-01 至 1998-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9724999&HistoricalAwards=false
• 关键词: SGER; Efficient; Extrachromosomal; Replication; Exogenous

Long 9724999 This Small Grant for Exploratory Research is based on the discovery that the filamentous fungus, Pestalotiopsis microspora, has a unique ability to convert non-replicating transforming DNA into self-replicating plasmids. This is in stark contrast, in terms of both efficiency and mechanism, to essentially all other filamentous fungi which replicate transforming DNA through chromosomal integration. Two aspects of P. microspora transformation, high efficiency and generation of self-replicating plasmids, could, if applicable to other filamentous fungi, have substantial impact on the growing field of molecular genetic engineering of this important class of organisms. This research develops methods for amplification of P. microspora plasmids, structural and functional characterization of these plasmids, and experiments to test their ability to replicate in other filamentous fungi.

Long 9724999这项探索性研究的小额赠款是基于发现丝状真菌Pestalotiopsis microspora具有将非复制转化DNA转化为自我复制质粒的独特能力。 就效率和机制而言，这与基本上所有其他通过染色体整合复制转化DNA的丝状真菌形成鲜明对比。 小孢原毛平革菌转化的两个方面，即高效率和自我复制质粒的产生，如果适用于其他丝状真菌，可能会对这类重要生物的分子遗传工程的日益增长的领域产生重大影响。 本研究开发了用于扩增P. microspora质粒的方法，这些质粒的结构和功能表征，以及测试其在其他丝状真菌中复制能力的实验。