AB14: A Putative Transcription Factor Regulating Arabidopsis Seed ABA Response

9728297 Finkelstein植物激素脱落酸（阿坝）调节影响作物生产力的植物生长和发育的许多方面，包括胚胎发生、水分关系和对各种环境胁迫的耐受性。 我们的长期目标是研究参与阿坝反应的元件的功能和相互作用。 Finkelstein博士最近克隆了种子对阿坝反应所需的基因AB 14，并发现它编码一种与一类转录因子同源的蛋白质。 本提案中概述的实验将表征植物内AB 14合成的位置和时间，其生化功能，并研究其与其他基因产物在协调种子发育中的相互作用。 我们的假设是，AB 14是一个小家族的转录因子，其中至少有一个（AB 14）参与种子和/或ABA的基因表达调控，可能与其他转录因子的种子特异性成员。 PI希望这些研究对了解该基因家族其他成员的活性具有普遍意义。 此外，它们可能具有潜在的操纵种子营养和贮藏品质的实际意义。

9728297 Finkelstein The plant hormone abscisic acid (ABA) regulates many aspects of plant growth and development affecting crop productivity, including embryogenesis, water relations and tolerance of a variety of environmental stresses. Our long term goal is to study the function and interaction of elements involved in responses to ABA. Dr. Finkelstein has recently cloned a gene required for seed responses to ABA, AB14, and found it to encode a protein with homology to a class of transcription factors. The experiments outlined in this proposal will characterize the location and timing of AB14 synthesis within the plant, its biochemical function, and investigate its interactions with other gene products in coordinating seed development. Our hypothesis is that AB14 is a seed-specific member of a small family of transcription factors, at least one of which (AB14) participates in seed- and/or ABA-regulation of gene expression, possibly in combination with other transcription factors. The PI expects these studies to have general relevance to understanding the activities of the other members of this gene family. Furthermore, they may have practical significance for potential manipulation of seed nutritional and storage qualities.