AB15: A bZIP Protein Regulating ABA Response

AB15：调节 ABA 反应的 bZIP 蛋白

• 批准号: 9982779
• 负责人: Ruth Finkelstein
• 金额: --
• 依托单位: University of California-Santa Barbara
• 依托单位国家: 美国
• 项目类别: Continuing grant
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-04-01 至 2004-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9982779&HistoricalAwards=false
• 关键词: AB15; bZIP; Protein; Regulating; ABA

The plant hormone abscisic acid (ABA) regulates many aspects ofplant growth and development affecting crop productivity, includingembryogenesis, water relations and tolerance of a variety of environmentalstresses. Our long-term goal is to study the function and interaction ofelements involved in response to ABA. One gene required for normal ABAresponse, the Arabidopsis ABA-insensitive 5 (ABI5) locus, has beenidentified by positional cloning in our lab and found to encode a proteinwith homology to the bZIP-domain class of transcription factors. Theexperiments outlined in this proposal will characterize the expression andbiochemical function of ABI5, and investigate its interactions with othergene products in coordinating seed development and response to ABA. Ourhypothesis is that ABI5 participates in seed- and/or ABA-regulation of geneexpression, possibly in combination with other transcription factors. Thesestudies should have general relevance to understanding the signalingnetwork(s) regulating embryonic and hormonally regulated gene expression.Furthermore, they may have practical significance for potentialmanipulation of seed nutritional and storage qualities or vegetative stresstolerance.

植物激素脱落酸(ABA)调节植物生长发育的许多方面，影响作物生产力，包括胚胎发生、水分关系和对各种环境胁迫的耐受性。我们的长期目标是研究参与ABA反应的各种元素的功能和相互作用。我们实验室通过定位克隆鉴定了一个正常ABA反应所必需的基因，即拟南芥ABA不敏感基因5(ABI5)，并发现它编码一种与转录因子bZIP结构域同源的蛋白。本研究将对ABI5基因的表达和生化功能进行研究，并探讨其与其他基因产物在协调种子发育和对ABA反应中的相互作用。我们的假设是，ABI5可能与其他转录因子结合，参与种子和/或ABA对基因表达的调控。这些研究对于理解调控胚胎和激素调控基因表达的信号网络(S)具有普遍意义。此外，它们可能对种子营养和贮藏品质的潜在调控或营养逆境耐性的潜在调控具有实际意义。