Molecular Analysis of Arabidopsis Genes Involved in Responseto Abscisic Acid

脱落酸（ABA）调节植物生长的许多方面， 发展，但承认和 细胞反应仍然不清楚。芬克尔斯坦医生的总体情况 目的是鉴定参与ABA作用的蛋白质， 从生理生化和分子生物学角度研究其功能 遗传分析她用拟南芥的突变体 改变对ABA的敏感性，以鉴定与ABA有关的基因 反应芬克尔斯坦博士还分离出拟南芥基因组 与玉米ABA不敏感基因座vp1同源的克隆。她 建议分析其功能：1）确定是否 它们对应于任何ABI基因座; 2）表征 它们在两种野生型中表达的发育特异性 和abi植物; 3）DNA序列分析和4） 免疫定位如果两个序列都不与任何abi共分离， 基因座，她将尝试创建一个突变表型， 反义基因 %%% 植物生长调节剂脱落酸影响植物生长的许多方面， 植物生长和发育，包括胚胎发育， 种子休眠，水分关系，细胞伸长，耐盐性 各种环境压力和衰老。本研究将 确定一些与脱落酸作用有关的基因。

Abscisic acid (ABA) regulates many aspects of plant growth and development, but the chain of events between recognition and cellular response remains obscure. Dr. Finkelstein's overall objective is to identify proteins involved in ABA action and study their function by physiological, biochemical and molecular genetic analysis. She is using mutants of Arabidopsis with altered sensitivity to ABA to identify genes involved in ABA response. Dr. Finkelstein has also isolated Arabidopsis genomic clones with homology to the maize ABA-insensitive locus, vp1. She proposes to analyze their function by: 1) determining whether they correspond to any of the ABI loci; 2) characterizing the developmental specificity of their expression in both wild type and abi plants; 3) DNA sequence analysis and 4) immunolocalization. If neither sequence cosegregates with any abi locus, she will attempt to create a mutant phenotype using antisense genes. %%% THe plant growth regulator abscisic acid affects many aspects of a plants growth and development, including embryo development, seed dormancy, water relations, cell elongation, tolerance to a variety of environmental stresses and senescence. This study will identify some of the genes involved in abscisic acid action.