Molecular Analysis of Arabidopsis Genes Involved in Responseto Abscisic Acid

拟南芥对脱落酸反应相关基因的分子分析

• 批准号: 8903425
• 负责人: Ruth Finkelstein
• 金额: $ 13万
• 依托单位: University of California-Santa Barbara
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-09-01 至 1992-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8903425&HistoricalAwards=false
• 关键词: Molecular; Analysis; Arabidopsis; Genes; Involved

Abscisic acid (ABA) regulates many aspects of plant growth and development, but the chain of events between recognition and cellular response remains obscure. Dr. Finkelstein's overall objective is to identify proteins involved in ABA action and study their function by physiological, biochemical and molecular genetic analysis. She is using mutants of Arabidopsis with altered sensitivity to ABA to identify genes involved in ABA response. Dr. Finkelstein has also isolated Arabidopsis genomic clones with homology to the maize ABA-insensitive locus, vp1. She proposes to analyze their function by: 1) determining whether they correspond to any of the ABI loci; 2) characterizing the developmental specificity of their expression in both wild type and abi plants; 3) DNA sequence analysis and 4) immunolocalization. If neither sequence cosegregates with any abi locus, she will attempt to create a mutant phenotype using antisense genes. %%% THe plant growth regulator abscisic acid affects many aspects of a plants growth and development, including embryo development, seed dormancy, water relations, cell elongation, tolerance to a variety of environmental stresses and senescence. This study will identify some of the genes involved in abscisic acid action.

脱落酸（ABA）调节植物生长发育的许多方面，但识别和细胞反应之间的事件链尚不清楚。Finkelstein博士的总体目标是通过生理、生化和分子遗传分析来识别参与ABA作用的蛋白质并研究其功能。她正在使用对ABA敏感性改变的拟南芥突变体来鉴定与ABA反应有关的基因。芬克尔斯坦博士还分离出与玉米aba不敏感位点vp1同源的拟南芥基因组克隆。她建议通过以下方法来分析它们的功能：1)确定它们是否对应于任何ABI位点；2)分析了它们在野生型和abi植物中表达的发育特异性；3) DNA序列分析和4)免疫定位。如果两个序列都没有与abi位点共分离，她将尝试使用反义基因创建突变表型。植物生长调节剂脱落酸影响植物生长发育的许多方面，包括胚胎发育、种子休眠、水分关系、细胞伸长、对各种环境胁迫的耐受性和衰老。本研究将确定一些参与脱落酸作用的基因。