Improvement of Biochemistry Laboratories for Science Majors and Non-Majors

生物化学领域在过去的20年里发生了巨大的变化。 讲座很容易更新，以反映这些技术的改进和应用，但实验室练习往往缺乏相应的现代化。 由于实验室需要加强讲座的概念，教授技术和分析技能，并提供积极的和合作的学习，他们必须得到改善。 本计画为生物化学课程引进新的相关实验问题，供理工科与非理工科学生使用。 在公共基础课程中，引入了两个新的实验，重点是LDH同工酶和LDH cDNA在正常和疾病状态下的分析。 这些实验需要用于DNA扩增的PCR系统、用于DNA定量的简单荧光计以及用于蛋白质电泳和DNA SSCP分析的电泳设备。 本计画提供此等仪器以进行聚合酶链反应、单链构象多态性分析及等电聚焦分析，供理工科学生进行载脂蛋白E变异体分析之实验计画。 通过参与项目设计，科学专业的学生学习使用文献和蛋白质和DNA结构数据库。 选定的设备可以进一步修改项目设计，纳入现代电泳技术和使用计算机存取的信息。

The field of Biochemistry has changed significantly in the past 20 years. Lectures are easily updated to reflect these technological improvements and applications, but laboratory exercises often lack equivalent modernization. Because laboratories are needed to reinforce lecture concepts, to teach technical and analytical skills, and to provide active and cooperative learning, they must be improved. This project introduces new, relevant experimental problems for biochemistry courses servicing both science majors and nonmajors. In the elementary nonmajors course, two new experiments are introduced which focus on the analysis of LDH isoenzymes and LDH cDNAs in normal and disease states. These experiments require a PCR system for DNA amplification, a simple fluorometer for DNA quantitation, and electrophoresis equipment for protein electrophoresis and DNA SSCP analysis. This project provides these instruments to perform PCR, SSCP analysis, and isoelectric focusing in a laboratory project for science majors focused on the analysis of apolipoprotein E variants. Through their participation in the project design, science majors learn to use literature and protein and DNA structure databases. The equipment selected allows further modification in project designs that incorporate modern electrophoresis techniques and the use of computer-accessed information.