POWRE: Use of Fluorescent Oligonucleotide Probes & Other Advanced Techniques to Characterize Population Distribution of Nitrifying Bacteria

POWRE：荧光寡核苷酸探针的使用

• 批准号: 9753086
• 负责人: Linda Figueroa
• 金额: $ 7.49万
• 依托单位: Colorado School of Mines
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-10-01 至 2000-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9753086&HistoricalAwards=false
• 关键词: POWRE; Use; Fluorescent; Oligonucleotide; Probes

9753086 Figueroa The active population distribution of nitrifying bacteria is difficult to study using traditional cultivation techniques such as most probable number and selective plating. This is partially because nitrifying bacteria grow very slowly - replicating once every eight hours under optimal conditions compared to E. coli which replicates once every twenty minutes. Culturing techniques also require the disruption of the biofilm structure and a loss of spatial distribution information. One relatively new microbiological technique that can be applied to an intact biofilm sample is Fluorescent In-Situ Hybridization (FISH). The application of FISH to full-scale wastewater treatment systems is limited. This could yield important information that can be correlated with performance and then used to quantify the active population for process modeling and optimization. Other techniques such as confocal microscopy and microprobes may be applicable to intact biofilm samples collected from full-scale trickling filters and may provide additional information for process control. The main research objective is to investigate the application of relatively new microbiological and biofilm techniques to characterize intact biofilm samples and determine whether this information can be used to improve process modeling and optimization. Secondary objectives are to develop new skills and collaborative relationships to increase competitiveness for the use of these novel techniques to quantify phenotypes and their distribution in full-scale biofilm processes. ***

利用最可能数和选择性电镀等传统的培养技术，很难研究硝化细菌的活跃种群分布。部分原因是硝化细菌生长非常缓慢，在最佳条件下每8小时复制一次，而大肠杆菌每20分钟复制一次。培养技术也需要破坏生物膜结构和空间分布信息的丢失。一种相对较新的微生物技术，可以应用于一个完整的生物膜样品是荧光原位杂交（FISH）。FISH在全面污水处理系统中的应用是有限的。这可以产生与性能相关的重要信息，然后用于量化流程建模和优化的活动人口。其他技术，如共聚焦显微镜和微探针可能适用于从全尺寸滴滤器收集的完整生物膜样品，并可能为过程控制提供额外的信息。主要研究目的是研究相对较新的微生物学和生物膜技术的应用，以表征完整的生物膜样品，并确定这些信息是否可用于改进过程建模和优化。次要目标是发展新的技能和合作关系，以提高使用这些新技术来量化表型及其在全尺寸生物膜过程中的分布的竞争力。＊＊＊