Molecular ecological analysis of the pig's intestinal microbiota in response to fermentable carbohydrates and calcium-phosphate in the diet

可发酵碳水化合物，如非淀粉多糖(NSP)，被认为影响猪胃肠道(GIT)中本地微生物区系的组成、发育和活性。此外，通过增加饲料中钙磷(CAP)和低聚糖的结合水平，在大鼠中证明了乳酸菌的刺激作用，而对猪的数据仍然缺乏。细菌的活动反过来可能会改变肠道对矿物质的吸收程度。已知发酵最终产物可刺激肠道上皮的营养作用，主要是通过在猪胃肠道近端和远端产生丁酸。在回肠末端装有肠道插管的生长小车将被用来确定(I)高粘度和低粘度NSP和可发酵NSP和低可发酵NSP的影响，以及(Ii)不同的饲料帽水平与NSP结合对回肠和结肠细菌群落的分子多样性、发育和代谢活动的影响，特别是乳酸菌和丁酸盐的形成，以及对饲料钙和磷的肠道吸收的影响。交付成果包括制定改善肠道健康的营养战略和优化矿物质供应，受可发酵碳水化合物供应和生长猪日粮上限的影响。

Fermentable carbohydrates, such as non-starch polysaccharides (NSP), are considered to influence the composition, development, and activity of the indigenous microbiota in the gastrointestinal tract (GIT) of pigs. Moreover, stimulation of lactobacilli was evidenced in rats by increasing the dietary calcium-phosphate (CaP) level in combination with oligosaccharides, while data are still missing for pigs. Bacterial activity, in turn, may change the degree of mineral absorption from the gut. Fermentation end products are known to stimulate trophic effects on the intestinal epithelium, mainly through butyrate production in the proximal and distal parts of the GIT of pigs. Growing barrows, fitted with intestinal cannulas at the distal ileum, will be used to determine (i) the effect of high viscous versus low viscous NSP and fermentable versus low fermentable NSP, and (ii) the effect of varying dietary CaP levels in combination with NSP on the molecular diversity, development and metabolic activity of the ileal and colonic bacterial community, with special view on lactic acid bacteria and butyrate formation, as well as on the intestinal absorption of dietary calcium and phosphorus. Deliverables include the development of nutritional strategies for improvement of gut health and optimization of mineral supply as influenced both by the supply of fermentable carbohydrates and CaP in diets for growing pigs.