Molecular ecological study for conservation of endangered species, Pyrus calleryana Decne var. dimorphopheylla (Makino) Koidz. : Genetic analysis on population structure, gene flow and reproductive deterioration

濒危物种保护的分子生态研究,Pyrus calleryana Decne var.

基本信息

  • 批准号:
    18580142
  • 负责人:
  • 金额:
    $ 2.38万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
  • 财政年份:
    2006
  • 资助国家:
    日本
  • 起止时间:
    2006 至 2007
  • 项目状态:
    已结题

项目摘要

In order to obtain genetic information for conservation of Pyrus calleryana Decne var. dimorphopheylla (Makino) Koidz., the endangered species, all of the individuals exist in Japan were sampled and inquired their origin, revealed that total number of individuals is 465 and they distributed only in Aich and Mie Prefecture. Genotypes of them were determined with ten nuclear SSR markers. Genetic diversity was maintained in this species, however, patterns of "isolation by distance" was not clear among all populations containing those affected by serious human impacts, such as, planting and transplanting. In contrast, genetic structure among the native populations was conserved. Phylogenetic tree and Bayesian cluster analysis revealed that the native populations were divided into two clusters, those are Aichi and Mie. Analyzing the nucleotide sequences for cDNAs corresponding to the alleles of the self-incompatible locus revealed eleven different alleles. Using the specific primers designed for each of S-allele, S-genotypes for 247 individuals collected from ten populations were determined and revealed that four to ten S-alleles were maintained in each population. Paternal analysis for seeds collected from four mother trees in the isolated population was performed with nine SSR markers and revealed that pollen parents were widely distributed in the population. Ecological and genetic factors which affect male contribution was analyzed with generalized linear mixed model (GLMM) and revealed that distance from the mother is the most significant factor for male reproductive success. Although self-incompatibility was functioned in the mating event within the population, degree of S-genotype identity between parents did not strongly affect pollen mediated gene dispersal.These results will provide important information for planning conservation programs of this species.
为获得梨属(Pyrus calleryana Decne var. dimorphophheylla (Makino) Koidz)的遗传信息。对日本境内全部存在的濒危物种进行了抽样调查,结果表明,总个体数为465只,仅分布于爱齐县和三重县。用10个SSR标记确定了它们的基因型。该物种保持了遗传多样性,但在包含受严重人类影响(如种植和移植)影响的所有种群中,“距离隔离”模式并不明显。而本地居群的遗传结构则较为保守。系统进化树和贝叶斯聚类分析表明,本地居群可划分为爱知和Mie两个聚类。分析自交不亲和位点对应的cdna核苷酸序列,发现了11个不同的等位基因。利用为每个s等位基因设计的特异性引物,对来自10个群体的247个个体的s基因型进行了测定,结果表明,每个群体中保持有4 ~ 10个s等位基因。对分离群体中4棵母树的种子进行了9个SSR标记的父系分析,结果表明花粉亲本在群体中分布广泛。利用广义线性混合模型(GLMM)分析了影响雄性贡献的生态因素和遗传因素,发现与母亲的距离是影响雄性繁殖成功的最重要因素。虽然自交不亲和在群体内的交配事件中起作用,但亲本间s基因型的同源程度对花粉介导的基因传播没有强烈影响。这些结果将为该物种的保护规划提供重要信息。

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
絶滅危惧種マメナシにおける種子の生産性と自家不和合性
濒危梨品种的种子生产力和自交不亲和性
  • DOI:
  • 发表时间:
    2006
  • 期刊:
  • 影响因子:
    0
  • 作者:
    加藤珠理;岩本祐佳;松岡美帆;向井譲
  • 通讯作者:
    向井譲
Species in Red List. 52. Pyrus calleryana Decnevar. dimorphopheylla (Makino) Koidz. (in Japanese)
红色名录中的物种。
  • DOI:
  • 发表时间:
    2008
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Kato;S.
  • 通讯作者:
    S.
Genetic diversity and reproductive capacity in the natural populations Pyrus of calleryana
自然种群的遗传多样性和繁殖能力
  • DOI:
  • 发表时间:
    2008
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Nisioka;R Kato;S. Imai;J.. Mukai;Y
  • 通讯作者:
    Y
絶滅危惧種マメナシ自生個体群の遺伝的多様性と繁殖能力
濒危豆梨本地种群的遗传多样性和繁殖能力
  • DOI:
  • 发表时间:
    2008
  • 期刊:
  • 影响因子:
    0
  • 作者:
    西岡理絵;加藤珠理;今井淳;向井譲
  • 通讯作者:
    向井譲
絶滅危惧種マメナシにおける遺伝構造の解明
阐明濒危物种豆梨的遗传结构
  • DOI:
  • 发表时间:
    2008
  • 期刊:
  • 影响因子:
    0
  • 作者:
    今井淳;加藤珠理;向井譲
  • 通讯作者:
    向井譲
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MUKAI Yuzuru其他文献

MUKAI Yuzuru的其他文献

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{{ truncateString('MUKAI Yuzuru', 18)}}的其他基金

Reproductive interference of Chamaecyparis pisifera by the heterospecific pollens from artificial plantation of Cryptomeria japonica and Chamaecyparis obtusa
人工林柳杉和扁柏异种花粉对扁柏生殖的干扰
  • 批准号:
    26450190
  • 财政年份:
    2014
  • 资助金额:
    $ 2.38万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Investigations on the mechanism for avoidance of selfing and its ecological meaning in nut production of Fagaceae
壳斗科坚果生产避免自交机制及其生态意义研究
  • 批准号:
    21580179
  • 财政年份:
    2009
  • 资助金额:
    $ 2.38万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Investigation on the factors for depression of propagation ability in an isolated beech population-Analysis of the loci for inbreeding depression maintained within a meta-population-
山毛榉孤立种群繁殖能力下降的因素调查-集合种群内近交衰退位点分析-
  • 批准号:
    15380101
  • 财政年份:
    2003
  • 资助金额:
    $ 2.38万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Study on the photoprotective functions of rhodoxanthin accumulated in the needles of Cryptomeria japonica and Chamaecyparis obtusa
柳杉和日本扁柏针叶中积聚的红松黄素的光保护作用研究
  • 批准号:
    12460067
  • 财政年份:
    2000
  • 资助金额:
    $ 2.38万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Study on the Evaluation of the Abilities for Protection Against the Ultraviolet B Radiation in the Forest Tree Species.
林木树种抗紫外线B辐射能力评价研究。
  • 批准号:
    09660159
  • 财政年份:
    1997
  • 资助金额:
    $ 2.38万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)

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