Generation of breeders for setting up mutant mouse colonies by priority programme members

这个中央服务项目产生和维护与肥大细胞研究相关的突变小鼠。正在并将向优先方案的感兴趣的参与者提供来自几个突变小鼠群体的繁殖者对和有限数量的后代，以及关于如何与这些小鼠合作的建议和指导。具体地说，缺乏肥大细胞(MC)的天然小鼠突变体将被培育并保存在柏林夏洛特大学皮肤病和过敏学系的小鼠设施中。这些突变体包括WBB6F1-KitW/W-v(W/Wv)小鼠、C57BL/6-KitW-sh/W-sh(Sash)小鼠和WCB6F1-KitS1/Sl-d(S1/SLD)小鼠。同时，德累斯顿大学免疫学研究所的小鼠设施将产生和保持几个基于Cre/loxP的突变系的小鼠群体，包括肥大细胞特异性Cre表达系、各种基因MC特异性失活的小鼠、诱导性或结构性MC缺乏症的新模型以及MC表达荧光蛋白的MC报告株。这些小鼠突变体对于鉴定和表征新的MC功能是必不可少的。优先计划中的大多数项目在某一时刻将需要使用这种突变小鼠。由于这些MICE不容易从商业或其他来源获得，这一服务项目将极大地促进这一优先计划的大多数成员的工作。此外，这一中央服务项目将大大减少为该计划的项目获得这些MICE所涉及的时间和成本。最后，为这一核心设施作出贡献的两个中心的现有专业知识将使所有致力于突变小鼠的项目从这一中央服务项目中受益，因为申请者及其团队为使用MC小鼠模型在体内设计和运行提供建议和方案。

This Central Service Project generates and maintains mutant mice that are relevant for mast cell research. Breeder pairs and limited numbers of offspring from several mutant mouse colonies as well as advice and guidance on how to work with these mice is and will be provided to interested participants of the priority programme. Specifically, natural mouse mutants that are deficient for mast cells (MCs) will be bred and kept at the mouse facilities of the Department of Dermatology and Allergy at Charité – Universitätsmedizin Berlin. These mutants include WBB6F1-KitW/W-v (W/Wv) mice, C57BL/6-KitW-sh/W-sh (sash) mice and WCB6F1-KitSl/Sl-d (Sl/Sld) mice. In parallel, the mouse facilities of the Institute of Immunology at TU-Dresden will generate and keep colonies of several lines of Cre/loxP-based mouse mutants including mast cell-specific Cre-expressing lines, mice with MC-specific inactivation of various genes, novel models of inducible or constitutive MC deficiency and MC reporter strains in which MCs express fluorescent proteins. These mouse mutants are essential for the identification and characterisation of novel MC functions. The majority of projects within the priority program will, at some point, require the use of such mutant mice. As these mice are not readily available from commercial or other sources, this service project will greatly facilitate the work of most members of this priority program. In addition, this central service project will significantly cut the time and costs involved in obtaining these mice for the projects of the program. Finally, the expertise available at the two centres that contribute to this core facility will have all projects working with mutant mice benefit from this central service projects, as the applicants and their teams provide advice and protocols for designing and running in vivo using MC mouse models.