Generation of breeders for setting up mutant mouse colonies by priority programme members

这个中心服务项目产生和维持与肥大细胞研究相关的突变小鼠。将向优先方案的感兴趣的参与者提供育种对和来自几个突变小鼠群体的有限数量的后代以及关于如何与这些小鼠一起工作的建议和指导。具体而言，将在Charité - Universitätsmedizin柏林的皮肤病和变态反应系的小鼠设施中繁殖并饲养肥大细胞（MC）缺陷的天然小鼠突变体。这些突变体包括WBB 6 F1-KitW/W-v（W/Wv）小鼠、C57 BL/6-KitW-sh/W-sh（sash）小鼠和WCB 6 F1-KitSl/Sl-d（Sl/Sld）小鼠。与此同时，德累斯顿工业大学免疫学研究所的小鼠设施将产生并保持几种基于Cre/loxP的小鼠突变体的菌落，包括肥大细胞特异性Cre表达系、各种基因MC特异性失活的小鼠、诱导型或组成型MC缺陷的新模型以及MC表达荧光蛋白的MC报告菌株。这些小鼠突变体对于鉴定和表征新型MC功能是必不可少的。优先计划中的大多数项目在某些时候都需要使用这种突变小鼠。由于这些小鼠不容易从商业或其他来源获得，这项服务项目将大大促进这一优先计划的大多数成员的工作。此外，这个中央服务项目将大大减少为该计划的项目获得这些鼠标所需的时间和成本。最后，这两个中心提供的专业知识将使所有与突变小鼠相关的项目受益于这一中心服务项目，因为申请人及其团队为使用MC小鼠模型的体内设计和运行提供建议和方案。

This Central Service Project generates and maintains mutant mice that are relevant for mast cell research. Breeder pairs and limited numbers of offspring from several mutant mouse colonies as well as advice and guidance on how to work with these mice is and will be provided to interested participants of the priority programme. Specifically, natural mouse mutants that are deficient for mast cells (MCs) will be bred and kept at the mouse facilities of the Department of Dermatology and Allergy at Charité – Universitätsmedizin Berlin. These mutants include WBB6F1-KitW/W-v (W/Wv) mice, C57BL/6-KitW-sh/W-sh (sash) mice and WCB6F1-KitSl/Sl-d (Sl/Sld) mice. In parallel, the mouse facilities of the Institute of Immunology at TU-Dresden will generate and keep colonies of several lines of Cre/loxP-based mouse mutants including mast cell-specific Cre-expressing lines, mice with MC-specific inactivation of various genes, novel models of inducible or constitutive MC deficiency and MC reporter strains in which MCs express fluorescent proteins. These mouse mutants are essential for the identification and characterisation of novel MC functions. The majority of projects within the priority program will, at some point, require the use of such mutant mice. As these mice are not readily available from commercial or other sources, this service project will greatly facilitate the work of most members of this priority program. In addition, this central service project will significantly cut the time and costs involved in obtaining these mice for the projects of the program. Finally, the expertise available at the two centres that contribute to this core facility will have all projects working with mutant mice benefit from this central service projects, as the applicants and their teams provide advice and protocols for designing and running in vivo using MC mouse models.