Sequencing of Arabidopsis Chromosome 2 (and beyond), and Development of Resources for Arabidopsisgenome Analysis

9813586 小型十字花科植物拟南芥已成为研究实验室中使用最广泛的实验模型行星系统。 它的小尺寸和快速生长周期与具有很少重复元件的小二倍体基因组相结合，使其成为研究植物生物学许多方面的理想模型系统。 自1990年以来，一直在进行准备工作，以开发研究其基因组所需的资源。 1996年，TIGR是美国资助启动拟南芥基因组测序的三个小组之一。 他们最初的测序工作集中在2号染色体上，并产生了超过3.3Mb的含有超过750个基因的最终注释序列，还有4.5Mb的测序克隆正在进行中，并产生了大量用于选择测序克隆的BAC末端序列。 TIGR还领导了关于注释对该项目重要性的讨论，并为拟南芥基因组序列制定了更统一的注释格式。 该项目计划将测序能力提高到每年约12 Mb，并利用这一能力完成2号染色体的测序，并与其他测序小组协调对基因组的其他区域进行测序。 除了继续进行基因组测序外，TIGR还提出从兰茨贝格直立藻中产生5,000个序列片段，用于鉴定多态性，从而用于精细定位突变的标记。 最后，为了解决拟南芥社区目前面临的注释标准的变化以及由于数据库快速变化而导致注释过时的问题，TIGR将建立整个拟南芥基因组的注释资源。 该资源将更新整个基因组的注释，以统一和信息丰富的方式呈现。 数据格式将与拟南芥数据库AtDB合作开发，以便结果可以与研究社区使用的丰富的非序列数据相关联。 测序能力的提高与这两个资源的建立相结合，将进一步加速我们对拟南芥的理解，从而从总体上理解植物生物学。

9813586 The small crucifer, Arabidopsis thaliana, has become the most widely used experimental model planet system used in research laboratories. Its small size and rapid growth cycle combined with a small, diploid genome with few repetitive elements make it an ideal model system for studying many aspects of plant biology. Since 1990, preparative work has been underway to develop the resources needed to study its genome. In 1996, TIGR was one of the three US groups funded to initiate genome sequencing on Arabidopsis. Their initial sequencing efforts have concentrated on chromosome 2 and have generated over 3.3Mb of finished annotated sequence containing over 750 genes, a further 4.5 mb of sequencing clones in progress, and have produced a large number of BAC end sequences used for the selection of sequencing clones. TIGR has also led the discussion on the importance of annotation to this project and developed guidelines for a more uniform annotation format for Arabidopsis genomic sequences. It is planned in this project to increase the sequencing capacity to approximately 12 Mb per year and use this capacity to complete the sequencing of chromosome 2, and to sequence in other regions of the genome in coordination with the other sequencing groups. In addition to the continued genomic sequencing, TIGR proposes to generate 5,000 sequence fragments from Landsberg erecta for use in identifying polymorphisms and therefore markers for fine mapping mutations. Finally, to address the variation in annotation standards currently facing the Arabidopsis community and the problem of outdated annotation due to rapid database changes, TIGR will establish an annotation resource for the entire Arabidopsis genome. This resource will update annotation for the entire genome, presenting it in a uniform and informative manner. The data format will be developed in collaboration with the Arabidopsis database, AtDB, so that the results can be linked to the wealth of non-sequence data used by the research community. The increase in sequencing capacity combined with the establishment of these two resources will further accelerate our understanding of Arabidopsis thaliana and therefore plant biology in general.