Dynamics of interphase chromosome territories and occurrence of homologous pairing of Arabidopsis chromosomes

利用染色体特异性BAC重叠群的FISH技术成功地对拟南芥整条染色体和近缘种的同源染色体进行了差异染色。基于这一前提，对两种染色体数不同的拟南芥物种（A. thaliana，x=5和A. arenosa，x-8）应该首次揭示整倍体植物中CT的空间排列和动态。特别是，体细胞同源配对的发生和潜在的动力学将澄清随机CT安排和准时同源配对的模型模拟的实验结果进行比较。突变体、诱变剂处理和转基因品系将用于测试改良重组行为和DNA损伤与间期CT的结构和排列的潜在相关性，如从随机模拟和同源体相互易位的频率预测的。

Differential painting of entire Arabidopsis thaliana chromosomes and of homeologous chromosomes of related species by FISH with chromosome-specific BAC contiges has been established successfully. Based on this prerequisite, painting of interphase chromosome territories (CTs) and FISH with chromosome-specific sequences on isolated nuclei of different cell cycle and developmental stages of two Arabidopsis species with different chromosome numbers (A. thaliana, x=5 and A. arenosa, x-8) should reveal for the first time the spatial arrangement and dynamics of CTs in euploid plants. In particular, the occurrence and potential dynamics of somatic homologous pairing will be clarified by comparison of the experimental results with model simulations of random CT arrangements and punctual homologous pairing. Mutants, mutagen treatments and transgenic lines will be applied to test for potential correlations of modified recombination behaviour and of DNA damage with the structure and arrangement of interphase CTs as predicted from random simulations and by the frequency of homologopus reciprocal translocations.