Dynamics of interphase chromosome territories and occurrence of homologous pairing of Arabidopsis chromosomes

利用染色体特异性BAC序列成功建立了拟南芥全染色体和近缘种同源染色体的FISH鉴别图。在此基础上，对染色体数目不同的拟南芥（拟南芥，x=5）和拟南芥（A. thaliana, x= 8）不同细胞周期和发育阶段的离体细胞核进行间期染色体区域（CTs）和具有染色体特异性序列的FISH绘制，将首次揭示整倍体植物中CTs的空间排列和动态。特别是，体细胞同源配对的发生和潜在动力学将通过实验结果与随机CT排列和准时同源配对的模型模拟进行比较来阐明。突变体、诱变剂处理和转基因系将被用于测试修饰重组行为和DNA损伤与间期ct的结构和排列的潜在相关性，这是根据随机模拟和同源互易位的频率预测的。

Differential painting of entire Arabidopsis thaliana chromosomes and of homeologous chromosomes of related species by FISH with chromosome-specific BAC contiges has been established successfully. Based on this prerequisite, painting of interphase chromosome territories (CTs) and FISH with chromosome-specific sequences on isolated nuclei of different cell cycle and developmental stages of two Arabidopsis species with different chromosome numbers (A. thaliana, x=5 and A. arenosa, x-8) should reveal for the first time the spatial arrangement and dynamics of CTs in euploid plants. In particular, the occurrence and potential dynamics of somatic homologous pairing will be clarified by comparison of the experimental results with model simulations of random CT arrangements and punctual homologous pairing. Mutants, mutagen treatments and transgenic lines will be applied to test for potential correlations of modified recombination behaviour and of DNA damage with the structure and arrangement of interphase CTs as predicted from random simulations and by the frequency of homologopus reciprocal translocations.