New Reagents for Asymmetric Organic Synthesis from Engineered Baker's Yeast

利用工程面包酵母进行不对称有机合成的新试剂

• 批准号: 9816318
• 负责人: Jon Stewart
• 金额: $ 33.75万
• 依托单位: University of Florida
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-01-01 至 2002-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9816318&HistoricalAwards=false
• 关键词: New; Reagents; Asymmetric; Organic; Synthesis

One focus of this research will be to alter the enantioselectivities of baker's yeast reductions. The resulting "designer yeasts" will be used for the synthesis of chiral building blocks and for a short, asymmetric synthesis of N-benzoyl-2- (2R,3S)-3-phenylisoserine, the beta-amino acid found in the side chain of Taxol. A second focus will employ a gene shuffling approach to improving the enantioselectivity of flavin monooxygenases.With this renewal award, the Organic and Macromolecular Chemistry Program is supporting the research of Dr. Jon D. Stewart of the Department of Chemistry at the University of Florida. Professor Stewart will focus his work on developing a synthetic strategy that combines the advantages of using purified enzymes with the experimental convenience of reactions using whole microbial cells. Specific enzymes are produced in whole cells of baker's yeast by DNA techniques. The engineered yeast strains, known as "designer yeasts," are then used to carry out synthetic reactions. New screening techniques for discovering enzymes with high enantioselectivity will also be developed.

本研究的一个重点将是改变面包酵母还原的对映体选择性。 由此产生的“设计师酵母”将用于手性结构单元的合成和N-苯甲酰基-2-（2 R，3S）-3-苯基异丝氨酸（紫杉醇侧链中发现的β-氨基酸）的短的不对称合成。 第二个重点将采用基因改组的方法来提高黄素单加氧酶的对映体选择性。有了这个更新的奖项，有机和大分子化学计划是支持博士的研究。佛罗里达大学化学系的斯图尔特说。 斯图尔特教授将把他的工作重点放在开发一种合成策略上，该策略将使用纯化酶的优势与使用整个微生物细胞的实验便利性相结合。 通过DNA技术在面包酵母的整个细胞中产生特定的酶。 然后，被称为“设计师酵母”的工程酵母菌株被用于进行合成反应。 还将开发用于发现具有高对映体选择性的酶的新筛选技术。