Engineering Baker's Yeast to Perform Enantioselective Oxidations and Applications to Organic Synthesis

本研究的重点是利用酵母菌株表达酶 在有机合成中的通用性。特定的氧化酶 待研究的包括环己酮单加氧酶、黄素单加氧酶 和细胞色素P-450这些酶不需要辅因子， 是手性间氯过氧苯甲酸的等价物， 将和非手性试剂本身一样容易使用。他们将 适用于进行烯烃环氧化等转化， 硫醚氧化（手性亚砜）和Baeyer-Villiger 反应.有了这个奖项，合成有机计划正在支持 Jon D博士的研究。斯图尔特的化学系的 佛罗里达大学。斯图尔特教授将把他的工作重点放在 设计用于进行对映选择性反应的酵母表达酶 在有机合成中。除了形成光学活性产物外， 其可用于制备立体化学复合体系， 这些酶都具有重要的生物学特性， 分子氧，而不是有机氧化剂， 从经济和环境的角度来看，

The focus of this research is the use of yeast strains to express enzymes of general utility in organic synthesis. The specific oxidative enzymes to be studied include cyclohexanone monooxygenase, flavin monooxygenase and cytochrome P-450's. These enzymes will not require cofactors and will be the equivalent of a chiral m-chloroperoxybenzoic acid in a form that will be as easy to use as the achiral reagent itself. They will be applicable to carry out such transformations as olefin epoxidations, thioether oxidations (to chiral sulfoxides) and Baeyer-Villiger reactions. With this award the Synthetic Organic Program is supporting the research of Dr. Jon D. Stewart of the Department of Chemistry of the University of Florida. Professor Stewart will focus his work on yeast-expressed enzymes designed to carry out enantioselective reactions in organic synthesis. Besides formation of optically active products which are useful for the preparation of stereochemically complex systems that have important biological properties, these enzymes all use molecular oxygen, rather than an organic oxidant, which makes them attractive from both an economic and environmental standpoint