Engineering Baker's Yeast to Perform Enantioselective Oxidations and Applications to Organic Synthesis

工程面包酵母进行对映选择性氧化及其在有机合成中的应用

• 批准号: 9513349
• 负责人: Jon Stewart
• 金额: $ 28.3万
• 依托单位: University of Florida
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-01-01 至 1998-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9513349&HistoricalAwards=false
• 关键词: Engineering; Baker; Yeast; Perform; Enantioselective

The focus of this research is the use of yeast strains to express enzymes of general utility in organic synthesis. The specific oxidative enzymes to be studied include cyclohexanone monooxygenase, flavin monooxygenase and cytochrome P-450's. These enzymes will not require cofactors and will be the equivalent of a chiral m-chloroperoxybenzoic acid in a form that will be as easy to use as the achiral reagent itself. They will be applicable to carry out such transformations as olefin epoxidations, thioether oxidations (to chiral sulfoxides) and Baeyer-Villiger reactions. With this award the Synthetic Organic Program is supporting the research of Dr. Jon D. Stewart of the Department of Chemistry of the University of Florida. Professor Stewart will focus his work on yeast-expressed enzymes designed to carry out enantioselective reactions in organic synthesis. Besides formation of optically active products which are useful for the preparation of stereochemically complex systems that have important biological properties, these enzymes all use molecular oxygen, rather than an organic oxidant, which makes them attractive from both an economic and environmental standpoint

这项研究的重点是使用酵母菌株来表达有机合成中通用的酶。待研究的具体氧化酶包括环己酮单加氧酶、黄素单加氧酶和细胞色素P-450。这些酶不需要辅因子，并且相当于手性间氯过苯甲酸，其形式与非手性试剂本身一样易于使用。它们将适用于进行烯烃环氧化、硫醚氧化（手性亚砜）和Baeyer-Villiger反应等转化。合成有机计划通过该奖项支持佛罗里达大学化学系 Jon D. Stewart 博士的研究。斯图尔特教授的工作重点是酵母表达的酶，这些酶旨在在有机合成中进行对映选择性反应。除了形成可用于制备具有重要生物特性的立体化学复杂系统的光学活性产物外，这些酶都使用分子氧，而不是有机氧化剂，这使得它们从经济和环境的角度来看都具有吸引力