RUI:The Paradox of Cellular Calcium Homeostasis During Vectorial Transfer: Spatial and Temporal Regulation of Ca Import/Export Proteins Using the Freshwater Crayfish Molting Model.

RUI：载体转移过程中细胞钙稳态的悖论：利用淡水小龙虾蜕皮模型对 Ca 进出口蛋白质的时空调节。

• 批准号: 0445202
• 负责人: Michele Wheatly
• 金额: --
• 依托单位: Wright State University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-06-01 至 2010-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0445202&HistoricalAwards=false
• 关键词: RUI; Paradox; Cellular; Calcium; Homeostasis

Calcium (Ca) is critical to many processes in living organisms. Calcium enters the body through specialized cells (epithelia, like the gut or gills); from that point it must be redirected as needs dictate away from regions of low concentration (cytosol or cell interior) towards regions of high use (for example biomineralization zones that are extracellular or outside of cells). Calcium is moved within and between cells across internal and external membranes through the activity of an array of Ca transporting proteins (Ca channels, pumps and exchangers). Calcium transporting epithelia present the ultimate paradox, namely, how can vast amounts of Ca move through the cell while cytosolic levels are kept low? Using cellular and molecular approaches, the freshwater crayfish molting model will be used to study how these Ca transporting proteins are regulated over time (comparing periods of high Ca flux that occur just before and after molting, with periods of Ca balance during intermolt periods). Spatial regulation will also be studied by comparing activity of proteins on internal vs. external membranes. The goal of this work is to better understand how arrays of different Ca transporting proteins are regulated to achieve cellular Ca homeostasis. This research will advance the basic understanding of membrane Ca transport which has the potential to impact many branches of biomedicine. It will also result in student training at both graduate and undergraduate levels at two primarily undergraduate institutions and will be translated into the K-12 curriculum that is consistent with National Science Education Standards.

钙（Ca）对生物体中的许多过程至关重要。 钙通过专门的细胞（上皮细胞，如肠道或鳃）进入体内;从那时起，它必须根据需要从低浓度区域（细胞溶质或细胞内部）转向高使用区域（例如细胞外或细胞外的生物矿化区）。 钙通过一系列钙转运蛋白（钙通道、泵和交换器）的活性在细胞内和细胞间穿过内外膜移动。 钙转运上皮细胞呈现出最终的矛盾，即，大量的钙如何在细胞内移动，而胞浆水平保持较低？ 使用细胞和分子的方法，淡水小龙虾蜕皮模型将被用来研究如何随着时间的推移，这些钙转运蛋白的调节（比较发生在蜕皮前后的高钙通量的时期，与蜕皮期间的钙平衡时期）。 还将通过比较内膜与外膜上的蛋白质活性来研究空间调节。 这项工作的目标是更好地了解不同的钙转运蛋白的阵列是如何调节，以实现细胞钙稳态。这项研究将推进对膜钙转运的基本理解，这有可能影响生物医学的许多分支。 它还将导致学生在两个主要的本科院校的研究生和本科水平的培训，并将被转化为K-12课程，是符合国家科学教育标准。