Acquistion of Equipment for Genomic Analysis Using Microarray Technology

购置使用微阵列技术进行基因组分析的设备

• 批准号: 9871386
• 负责人: Harold Riethman
• 金额: $ 10.14万
• 依托单位: Wistar Institute
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-09-15 至 2000-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9871386&HistoricalAwards=false
• 关键词: Acquistion; Equipment; Genomic; Analysis; Using

06/19/98 A major application of microarray technology is quantitative monitoring of gene expression patterns. Microarray experiments have been described recently in which dozens of new differentially expressed genes have been discovered using microdissected tissues and radioactive labeling and detection methods. Fluorescent detection methods afford the bast quantitation, permit co-hybridization of two samples labeled with different fluors to the same microarray grid, and permits resolution of very closely spaced microarrayed spots. However, it does have the drawback of increased expense and requires a much higher amount of starting material than radioactive detection methods. This proposal will support the purchase of instrumentation for a state-of-the-art facility that will use microarray technology for genomic analyses. Two instruments will be purchased: a confocal laser-scanning and imaging devise for reading high-density microarrays hybridized with probes labeled with fluorescent tags and a phosphoimager for high- resolution scanning of microarrays hybridized with probes labeled with radioisotopes. The research to be supported by this technology include combining microarray analysis with engineered transcriptional repressors for identification of target genes, fundamental studies of transcription regulation in yeast, studies of cellular pathways involved in transformation and apoptosis, novel approaches to elucidate the biological function of subnuclear cytological structures, studies of tissue regeneration and T-cell development in mice, analysis of subtelomeric variation and transcription patterns in humans, and the development of novel peptide-nucleic acid detection strategies. Graduate students and postdoctoral associates at the Wistar Institute will have access to this emerging technology, allowing them to incorporate this powerful new methodology into their research projects as they develop. More importantly, they will see the exciting new opportunities microarray analysis offers for understanding basic biological processes, and will begin to develop fresh approaches to biological problems that may have been seen as difficult or insoluble using non-global approaches.

06/19/98 微阵列技术的一个主要应用是基因表达模式的定量监测。 最近描述了微阵列实验，其中使用显微解剖组织和放射性标记和检测方法发现了数十种新的差异表达基因。 荧光检测方法提供了快速定量，允许用不同荧光标记的两个样品与相同的微阵列网格共杂交，并允许分辨率非常接近的微阵列点。 然而，它确实具有增加费用的缺点，并且比放射性检测方法需要更多量的起始材料。 该提案将支持为最先进的设施购买仪器，该设施将使用微阵列技术进行基因组分析。 将购买两台仪器：共焦激光扫描和成像设备，用于读取与荧光标签标记的探针杂交的高密度微阵列；以及磷光成像仪，用于对与放射性同位素标记的探针杂交的微阵列进行高分辨率扫描。 该技术支持的研究包括将微阵列分析与工程化转录抑制因子相结合以鉴定靶基因、酵母转录调控的基础研究、参与转化和凋亡的细胞途径研究、阐明亚核细胞结构生物学功能的新方法、小鼠组织再生和T细胞发育研究、亚端粒变异和转录模式分析。 人类，以及新型肽核酸检测策略的开发。 威斯塔研究所的研究生和博士后将能够接触到这项新兴技术，使他们能够在开发研究项目时将这种强大的新方法融入到他们的研究项目中。 更重要的是，他们将看到微阵列分析为理解基本生物过程提供的令人兴奋的新机会，并将开始开发新的方法来解决使用非全局方法可能被视为困难或无法解决的生物问题。