Chromatin Charting: Organization and Dynamics of Plant Nuclear DNA in situ
染色质图谱:植物核 DNA 的原位组织和动态
基本信息
- 批准号:9872636
- 负责人:
- 金额:$ 67.62万
- 依托单位:
- 依托单位国家:美国
- 项目类别:Standard Grant
- 财政年份:1998
- 资助国家:美国
- 起止时间:1998-10-01 至 2000-12-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
The nucleus is the subcellular organelle in which the bulk of the genomic information within an eukaryotic cell is organized. Although tremendous advances in sequencing genomes of organisms have taken place in the past few years, the way by which this vast amount of genetic information is stored and physically manipulated in the nuclei of plants and animals remains a mystery. From studies using hybridization technologies and microscopy work, a picture of an organized subnuclear structure has emerged. One major subnuclear structure is the chromosomes, which contain the genome of an organism. More recently, the application of the Green Fluorescent Protein (GFP) as an in vivo tag of chromosomal substances has allowed the visualization of chromosomes in live cells of animals and fungi. The goal for this project is to extend the GFP technology to understand subnuclear architecture by charting the relative physical position of specific genes on each of the chromosomes using cells within an intact plant. To achieve the objective of visualizing and charting the genes on all the chromosomes of a model plant, Arabidopsis, GFP and two different color variants (yellow [YFP] and blue [BFP]) of this protein will be used as in vivo tags in transgenic plants. Using the three distinct markers, the physical organization of the genome in relation to gene expression and gene mobilization patterns will be characterized. The project is a first step to establish this technology as a powerful tool to visualize the relationship between the genome organization and gene expression. While it is being tested in a model plant, Arabidopsis, this approach should provide the necessary resources and tools to address fundamental questions about subnuclear architecture and chromatin dynamics in all plants. Further, insights from this work should contribute to our understanding of global gene regulation as well as epigenetic phenomena in all eukaryotic organisms.
细胞核是亚细胞细胞器,真核细胞内的大量基因组信息在其中组织。尽管过去几年在生物体基因组测序方面取得了巨大进展,但动植物细胞核中大量遗传信息的存储和物理操作方式仍然是个谜。通过使用杂交技术和显微镜工作的研究,一幅有组织的亚核结构的图片已经出现。染色体是一种主要的亚核结构,它包含生物体的基因组。 最近,绿色荧光蛋白(GFP)作为染色体物质的体内标签的应用使得动物和真菌的活细胞中的染色体得以可视化。该项目的目标是通过使用完整植物内的细胞绘制每条染色体上特定基因的相对物理位置图来扩展 GFP 技术以了解亚核结构。为了实现对模型植物、拟南芥所有染色体上的基因进行可视化和绘制图表的目标,该蛋白的 GFP 和两种不同颜色的变体(黄色 [YFP] 和蓝色 [BFP])将被用作转基因植物的体内标签。使用三个不同的标记,将表征与基因表达和基因动员模式相关的基因组的物理组织。 该项目是将这项技术打造成可视化基因组组织和基因表达之间关系的强大工具的第一步。 虽然它正在模型植物拟南芥中进行测试,但这种方法应该提供必要的资源和工具来解决所有植物中亚核结构和染色质动力学的基本问题。此外,这项工作的见解应有助于我们理解所有真核生物的全球基因调控以及表观遗传现象。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
Eric Lam其他文献
SAFETY AND EFFICACY OF MARGIN AND BASE ABLATION AFTER ENDOSCOPIC MUCOSAL RESECTION OF LARGE NONPEDUNCULATED COLORECTAL POLYPS: A PROSPECTIVE MULTI-CENTER STUDY
内镜下黏膜切除大型无蒂结直肠息肉后切缘及基底消融的安全性和有效性:一项前瞻性多中心研究
- DOI:
10.1016/j.gie.2025.03.840 - 发表时间:
2025-05-01 - 期刊:
- 影响因子:7.500
- 作者:
Edgard Medawar;Mahsa Taghiakbari;Roupen Djinbachian;Neal C. Shahidi;John M. Levenick;Jeffrey D. Mosko;Eric Lam;Christopher Teshima;Matthew Moyer;Simon Bouchard;Érik Deslandres;Heiko Pohl;Daniel Von Renteln - 通讯作者:
Daniel Von Renteln
ENDOSCOPIC ULTRASOUND-GUIDED CHOLEDOCHODUODENOSTOMY: A LEARNING CURVE ANALYSIS BASED ON A MULTICENTRE RANDOMIZED CONTROLLED TRIAL (ELEMENT TRIAL)
- DOI:
10.1016/j.gie.2022.04.1260 - 发表时间:
2022-06-01 - 期刊:
- 影响因子:7.500
- 作者:
Yen-I Chen;Anand Sahai;Gianfranco Donatelli;Eric Lam;Sana Kenshil;Fergal Donnellan;Avijit Chatterjee;Nauzer Forbes;Jeffrey D. Mosko;Etienne Desilets;Sarto C. Paquin;Jennifer J. Telford;Gary R. May;Gurpal S. Sandha;Rachid Mohamed;S. Ian Gan;Andre Roy;Jad Abou Khalil;Corey S. Miller;Jeffrey Barkun;Alan Barkun - 通讯作者:
Alan Barkun
Structural determinants for pH-dependent activation of a plant metacaspase
植物类胱天蛋白酶 pH 依赖性激活的结构决定因素
- DOI:
10.1038/s41467-025-60253-y - 发表时间:
2025-05-29 - 期刊:
- 影响因子:15.700
- 作者:
Haijiao Liu;Max Henderson;Zhili Pang;Qingfang Zhang;Eric Lam;Qun Liu - 通讯作者:
Qun Liu
Programmed cell death, mitochondria and the plant hypersensitive response
程序性细胞死亡、线粒体与植物过敏反应
- DOI:
10.1038/35081184 - 发表时间:
2001-06-14 - 期刊:
- 影响因子:48.500
- 作者:
Eric Lam;Naohiro Kato;Michael Lawton - 通讯作者:
Michael Lawton
REAL WORLD EFFICACY OF A COLONIC SELECTIVE RESECTION ALGORITHM FOR LARGE NON-PEDUNCULATED COLON POLYPS
- DOI:
10.1016/j.gie.2024.04.2709 - 发表时间:
2024-06-01 - 期刊:
- 影响因子:
- 作者:
Shirley Jiang;Aein Zarrin;Angad Walia;Cherry Galorport;Robert Enns;Eric Lam;Neal Shahidi - 通讯作者:
Neal Shahidi
Eric Lam的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('Eric Lam', 18)}}的其他基金
Structure-Function Analysis of Type II Metacaspases to Reveal Distinct Activation Mechanisms
II 型元半胱天冬酶的结构功能分析揭示独特的激活机制
- 批准号:
2052997 - 财政年份:2021
- 资助金额:
$ 67.62万 - 项目类别:
Continuing Grant
Targeting the FOXM1 signature for early diagnosis and treatment in cholangiocarcinoma
靶向 FOXM1 特征用于胆管癌的早期诊断和治疗
- 批准号:
MR/N012097/1 - 财政年份:2016
- 资助金额:
$ 67.62万 - 项目类别:
Research Grant
Function and Regulation of Metacaspases in Plant Cell Death
后半胱天冬酶在植物细胞死亡中的功能和调节
- 批准号:
1258071 - 财政年份:2013
- 资助金额:
$ 67.62万 - 项目类别:
Continuing Grant
Meeting: International Conference on Duckweed Research at Rutgers University on August 21-24, 2013
会议:2013 年 8 月 21-24 日在罗格斯大学举行的浮萍研究国际会议
- 批准号:
1338642 - 财政年份:2013
- 资助金额:
$ 67.62万 - 项目类别:
Standard Grant
IGERT: Solutions for Renewable and Sustainable Fuels in the 21st Century
IGERT:21 世纪可再生和可持续燃料解决方案
- 批准号:
0903675 - 财政年份:2009
- 资助金额:
$ 67.62万 - 项目类别:
Continuing Grant
Functional Analysis of Metacaspases in Plant Development and Cell Death.
植物发育和细胞死亡中元半胱天冬酶的功能分析。
- 批准号:
0744709 - 财政年份:2008
- 资助金额:
$ 67.62万 - 项目类别:
Continuing Grant
Chromatin Charting: Organization and Dynamics of Plant Nuclear DNA in situ
染色质图谱:植物核 DNA 的原位组织和动态
- 批准号:
0077617 - 财政年份:2000
- 资助金额:
$ 67.62万 - 项目类别:
Continuing Grant
NSF Postdoctoral Research Fellowships in Plant Biology
NSF 植物生物学博士后研究奖学金
- 批准号:
8508764 - 财政年份:1985
- 资助金额:
$ 67.62万 - 项目类别:
Fellowship Award
相似海外基金
Charting the brain's wiring over the human lifespan
绘制人类一生中大脑的接线图
- 批准号:
DE240101035 - 财政年份:2024
- 资助金额:
$ 67.62万 - 项目类别:
Discovery Early Career Researcher Award
Charting the Development Sociotechnical Thinking in Engineering Students and Professionals
绘制工程专业学生和专业人员的社会技术思维发展图
- 批准号:
2332038 - 财政年份:2024
- 资助金额:
$ 67.62万 - 项目类别:
Standard Grant
Charting the human brain connectome over the lifespan
绘制人类大脑连接组在整个生命周期的图表
- 批准号:
FT220100091 - 财政年份:2023
- 资助金额:
$ 67.62万 - 项目类别:
ARC Future Fellowships
CAS-Climate: Workshop on Charting Net-Zero Carbon, Climate Resilient Transitions from Farms to Cities to National Scales: Learnings Across India and USA
CAS-气候:关于绘制净零碳、从农场到城市再到国家规模的气候适应转变的研讨会:印度和美国的经验教训
- 批准号:
2312305 - 财政年份:2023
- 资助金额:
$ 67.62万 - 项目类别:
Standard Grant
CUE-M: LEVEL UP: Charting a Pathway toward Inclusive Computing
CUE-M:LEVEL UP:绘制包容性计算之路
- 批准号:
2246079 - 财政年份:2023
- 资助金额:
$ 67.62万 - 项目类别:
Standard Grant
Charting subject-specific tau propagation along the gradients of brain connectivity in Alzheimer's disease
绘制阿尔茨海默病中特定受试者 tau 蛋白沿着大脑连接梯度的传播图
- 批准号:
479415 - 财政年份:2023
- 资助金额:
$ 67.62万 - 项目类别:
Operating Grants
Charting the fitness landscape of a penicillin-binding protein
绘制青霉素结合蛋白的适应度图
- 批准号:
MR/X007421/1 - 财政年份:2023
- 资助金额:
$ 67.62万 - 项目类别:
Fellowship
Conference: Charting & Expanding Needed Trails to Research Administration & Leadership (CENTRAL)
会议:制图
- 批准号:
2324469 - 财政年份:2023
- 资助金额:
$ 67.62万 - 项目类别:
Standard Grant
Charting a course towards new light-activated molecules
制定新的光激活分子的路线
- 批准号:
2881472 - 财政年份:2023
- 资助金额:
$ 67.62万 - 项目类别:
Studentship
Charting the evolutionary development of novel genes and the molecular mechanisms of gland tissue organizationin cephalopods
绘制头足类新基因的进化发展和腺体组织组织的分子机制
- 批准号:
10702230 - 财政年份:2023
- 资助金额:
$ 67.62万 - 项目类别: