Chromatin Charting: Organization and Dynamics of Plant Nuclear DNA in situ
染色质图谱:植物核 DNA 的原位组织和动态
基本信息
- 批准号:9872636
- 负责人:
- 金额:$ 67.62万
- 依托单位:
- 依托单位国家:美国
- 项目类别:Standard Grant
- 财政年份:1998
- 资助国家:美国
- 起止时间:1998-10-01 至 2000-12-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
The nucleus is the subcellular organelle in which the bulk of the genomic information within an eukaryotic cell is organized. Although tremendous advances in sequencing genomes of organisms have taken place in the past few years, the way by which this vast amount of genetic information is stored and physically manipulated in the nuclei of plants and animals remains a mystery. From studies using hybridization technologies and microscopy work, a picture of an organized subnuclear structure has emerged. One major subnuclear structure is the chromosomes, which contain the genome of an organism. More recently, the application of the Green Fluorescent Protein (GFP) as an in vivo tag of chromosomal substances has allowed the visualization of chromosomes in live cells of animals and fungi. The goal for this project is to extend the GFP technology to understand subnuclear architecture by charting the relative physical position of specific genes on each of the chromosomes using cells within an intact plant. To achieve the objective of visualizing and charting the genes on all the chromosomes of a model plant, Arabidopsis, GFP and two different color variants (yellow [YFP] and blue [BFP]) of this protein will be used as in vivo tags in transgenic plants. Using the three distinct markers, the physical organization of the genome in relation to gene expression and gene mobilization patterns will be characterized. The project is a first step to establish this technology as a powerful tool to visualize the relationship between the genome organization and gene expression. While it is being tested in a model plant, Arabidopsis, this approach should provide the necessary resources and tools to address fundamental questions about subnuclear architecture and chromatin dynamics in all plants. Further, insights from this work should contribute to our understanding of global gene regulation as well as epigenetic phenomena in all eukaryotic organisms.
细胞核是亚细胞器,真核细胞内的大部分基因组信息都是在细胞核中组织的。尽管在过去的几年里,在生物体基因组测序方面取得了巨大的进步,但这些海量的遗传信息是如何在动植物的细胞核中存储和物理操作的仍然是一个谜。从使用杂交技术和显微镜工作的研究中,出现了一幅有组织的亚核结构的图景。一个主要的亚核结构是染色体,它包含有机体的基因组。最近,绿色荧光蛋白(GFP)作为染色体物质的活体标记的应用使动物和真菌的活细胞中的染色体可视化成为可能。该项目的目标是利用完整植物中的细胞绘制特定基因在每条染色体上的相对物理位置图,从而扩展GFP技术以了解亚核结构。为了实现可视化和绘制模式植物所有染色体上的基因的目标,拟南芥、GFP和该蛋白的两个不同颜色变体(黄色[YFP]和蓝色[BFP])将被用作转基因植物的活体标签。使用这三个不同的标记,将描述与基因表达和基因动员模式相关的基因组的物理组织。该项目是将这项技术确立为可视化基因组组织和基因表达之间关系的强大工具的第一步。虽然这种方法正在拟南芥模式植物中进行测试,但它应该提供必要的资源和工具,以解决所有植物中关于亚核结构和染色质动力学的基本问题。此外,这项工作的见解应该有助于我们理解全球基因调控以及所有真核生物中的表观遗传现象。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Eric Lam其他文献
SAFETY AND EFFICACY OF MARGIN AND BASE ABLATION AFTER ENDOSCOPIC MUCOSAL RESECTION OF LARGE NONPEDUNCULATED COLORECTAL POLYPS: A PROSPECTIVE MULTI-CENTER STUDY
内镜下黏膜切除大型无蒂结直肠息肉后切缘及基底消融的安全性和有效性:一项前瞻性多中心研究
- DOI:
10.1016/j.gie.2025.03.840 - 发表时间:
2025-05-01 - 期刊:
- 影响因子:7.500
- 作者:
Edgard Medawar;Mahsa Taghiakbari;Roupen Djinbachian;Neal C. Shahidi;John M. Levenick;Jeffrey D. Mosko;Eric Lam;Christopher Teshima;Matthew Moyer;Simon Bouchard;Érik Deslandres;Heiko Pohl;Daniel Von Renteln - 通讯作者:
Daniel Von Renteln
ENDOSCOPIC ULTRASOUND-GUIDED CHOLEDOCHODUODENOSTOMY: A LEARNING CURVE ANALYSIS BASED ON A MULTICENTRE RANDOMIZED CONTROLLED TRIAL (ELEMENT TRIAL)
- DOI:
10.1016/j.gie.2022.04.1260 - 发表时间:
2022-06-01 - 期刊:
- 影响因子:7.500
- 作者:
Yen-I Chen;Anand Sahai;Gianfranco Donatelli;Eric Lam;Sana Kenshil;Fergal Donnellan;Avijit Chatterjee;Nauzer Forbes;Jeffrey D. Mosko;Etienne Desilets;Sarto C. Paquin;Jennifer J. Telford;Gary R. May;Gurpal S. Sandha;Rachid Mohamed;S. Ian Gan;Andre Roy;Jad Abou Khalil;Corey S. Miller;Jeffrey Barkun;Alan Barkun - 通讯作者:
Alan Barkun
Structural determinants for pH-dependent activation of a plant metacaspase
植物类胱天蛋白酶 pH 依赖性激活的结构决定因素
- DOI:
10.1038/s41467-025-60253-y - 发表时间:
2025-05-29 - 期刊:
- 影响因子:15.700
- 作者:
Haijiao Liu;Max Henderson;Zhili Pang;Qingfang Zhang;Eric Lam;Qun Liu - 通讯作者:
Qun Liu
Programmed cell death, mitochondria and the plant hypersensitive response
程序性细胞死亡、线粒体与植物过敏反应
- DOI:
10.1038/35081184 - 发表时间:
2001-06-14 - 期刊:
- 影响因子:48.500
- 作者:
Eric Lam;Naohiro Kato;Michael Lawton - 通讯作者:
Michael Lawton
REAL WORLD EFFICACY OF A COLONIC SELECTIVE RESECTION ALGORITHM FOR LARGE NON-PEDUNCULATED COLON POLYPS
- DOI:
10.1016/j.gie.2024.04.2709 - 发表时间:
2024-06-01 - 期刊:
- 影响因子:
- 作者:
Shirley Jiang;Aein Zarrin;Angad Walia;Cherry Galorport;Robert Enns;Eric Lam;Neal Shahidi - 通讯作者:
Neal Shahidi
Eric Lam的其他文献
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{{ truncateString('Eric Lam', 18)}}的其他基金
Structure-Function Analysis of Type II Metacaspases to Reveal Distinct Activation Mechanisms
II 型元半胱天冬酶的结构功能分析揭示独特的激活机制
- 批准号:
2052997 - 财政年份:2021
- 资助金额:
$ 67.62万 - 项目类别:
Continuing Grant
Targeting the FOXM1 signature for early diagnosis and treatment in cholangiocarcinoma
靶向 FOXM1 特征用于胆管癌的早期诊断和治疗
- 批准号:
MR/N012097/1 - 财政年份:2016
- 资助金额:
$ 67.62万 - 项目类别:
Research Grant
Function and Regulation of Metacaspases in Plant Cell Death
后半胱天冬酶在植物细胞死亡中的功能和调节
- 批准号:
1258071 - 财政年份:2013
- 资助金额:
$ 67.62万 - 项目类别:
Continuing Grant
Meeting: International Conference on Duckweed Research at Rutgers University on August 21-24, 2013
会议:2013 年 8 月 21-24 日在罗格斯大学举行的浮萍研究国际会议
- 批准号:
1338642 - 财政年份:2013
- 资助金额:
$ 67.62万 - 项目类别:
Standard Grant
IGERT: Solutions for Renewable and Sustainable Fuels in the 21st Century
IGERT:21 世纪可再生和可持续燃料解决方案
- 批准号:
0903675 - 财政年份:2009
- 资助金额:
$ 67.62万 - 项目类别:
Continuing Grant
Functional Analysis of Metacaspases in Plant Development and Cell Death.
植物发育和细胞死亡中元半胱天冬酶的功能分析。
- 批准号:
0744709 - 财政年份:2008
- 资助金额:
$ 67.62万 - 项目类别:
Continuing Grant
Chromatin Charting: Organization and Dynamics of Plant Nuclear DNA in situ
染色质图谱:植物核 DNA 的原位组织和动态
- 批准号:
0077617 - 财政年份:2000
- 资助金额:
$ 67.62万 - 项目类别:
Continuing Grant
NSF Postdoctoral Research Fellowships in Plant Biology
NSF 植物生物学博士后研究奖学金
- 批准号:
8508764 - 财政年份:1985
- 资助金额:
$ 67.62万 - 项目类别:
Fellowship Award
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