CAREER: Proteome Analysis in the Engineering of Escherichia coli with Enhanced Protein Secretion

本奖项的研究目标是：(1)结合数学模型和细胞工程策略，设计细菌细胞中的蛋白质分泌机制，以增强重组蛋白质产品的分泌；(2)建立研究生和本科生研究助理的综合团队文化来执行实验。这些小组的成员将酌情与来自其他学科和不同地点的研究人员开展对话。该奖项的教学目标是：(1)建立并提供一个基础设施，使工程本科学生接触和训练一系列工程技能，包括研究技术、结果交流和实际问题解决，特别强调培养和准备学生从事研究工作；(2)提供一个基础设施，其中研究生水平的工程学生接受跨学科方法的培训，重点是研究工业中遇到的实际问题，同时加强完成论文所需的研究技能。

The Research Objectives of this Award are to: (1) use a combination of mathematical models and cellular engineering strategies to engineer the protein secretion machinery in bacterial cells for enhanced secretion of a recombinant protein product; and (2) establish a culture of integrated teams of graduate and undergraduate research assistants to execute experiments. Members of these teams are to develop dialogues with researchers from other disciplines and different locations as appropriate. The Teaching Objectives of the Award are to: (1) establish and provide an infrastructure wherein undergraduate engineering students are exposed to and trained in a range of engineering skills including research techniques, communication of results, and practical problem solving with a particular emphasis on cultivating and preparing students for research careers; and (2) provide an infrastructure wherein graduate-level engineering students are trained in multidisciplinary approaches to interdisciplinary problems with an emphasis placed on studying practical problems encountered in industry while reinforcing the research skills necessary to complete a dissertation.