CAREER: Proteome Analysis in the Engineering of Escherichia coli with Enhanced Protein Secretion

职业：增强蛋白质分泌的大肠杆菌工程中的蛋白质组分析

• 批准号: 9874938
• 负责人: Kelvin Lee
• 金额: $ 30.92万
• 依托单位: Cornell University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-05-01 至 2004-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9874938&HistoricalAwards=false
• 关键词: CAREER; Proteome; Analysis; Engineering; Escherichia

The Research Objectives of this Award are to: (1) use a combination of mathematical models and cellular engineering strategies to engineer the protein secretion machinery in bacterial cells for enhanced secretion of a recombinant protein product; and (2) establish a culture of integrated teams of graduate and undergraduate research assistants to execute experiments. Members of these teams are to develop dialogues with researchers from other disciplines and different locations as appropriate. The Teaching Objectives of the Award are to: (1) establish and provide an infrastructure wherein undergraduate engineering students are exposed to and trained in a range of engineering skills including research techniques, communication of results, and practical problem solving with a particular emphasis on cultivating and preparing students for research careers; and (2) provide an infrastructure wherein graduate-level engineering students are trained in multidisciplinary approaches to interdisciplinary problems with an emphasis placed on studying practical problems encountered in industry while reinforcing the research skills necessary to complete a dissertation.

该奖项的研究目标是：（1）使用数学模型和细胞工程策略的组合来设计细菌细胞中的蛋白质分泌机制，以增强重组蛋白产品的分泌;（2）建立研究生和本科生研究助理的综合团队的文化，以执行实验。 这些小组的成员将酌情与来自其他学科和不同地点的研究人员开展对话。 该奖项的教学目标是：（1）建立和提供一个基础设施，其中本科工程专业的学生接触和培训的一系列工程技能，包括研究技术，结果的沟通，并特别强调培养和准备学生的研究生涯解决实际问题;（2）提供一个基础设施，使毕业生-一级工程专业的学生接受多学科方法的培训，以解决跨学科问题，重点是研究工业中遇到的实际问题，同时加强完成所需的研究技能。一篇论文