EAGER: The International CHO Genome Community Project

EAGER：国际 CHO 基因组社区项目

• 批准号: 1124627
• 负责人: Kelvin Lee
• 金额: $ 10万
• 依托单位: University of Delaware
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-08-01 至 2013-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1124627&HistoricalAwards=false
• 关键词: EAGER; International; CHO; Genome; Community

The overall objective for this EAGER proposal is to develop a framework to support the international Chinese hamster ovary (CHO) cell community's effort to move into the genomeera. CHO cells are currently the most widely used mammalian cell line for biopharmaceutical production. Nearly 70% of all recombinant biotherapeutics, yielding a global market of nearly $100 billion per year, are produced in CHO cells. Biopharmaceutical production will continue to provide the pharmaceutical industry with tremendous growth in the next several years, enabling continued research into new medical treatments and the expansion of manufacturing facilities. Unfortunately, efforts to improve the system using metabolic engineering and other scientific and engineering methodologies have lagged due to the absence of a genome and communitygenome framework. As a result, this project will implement the infrastructure to pursue two specific aims for support of the international CHO community:Specific Aim #1:Develop an information technology (IT) - infrastructure to support the sharing of genome-scale data and tools for the CHO biotechnology community and provide a forum where the CHO biotechnology community can interact and synergize on-line.Specific Aim #2:Educate graduate students on the use of bioinformatics to implement and analyze the CHO genome and enhance specific productivity and product quality using new webbased tools of importance to the CHO community.The intellectual merit of this proposal results from the development of a one-stop website portal to support the community. As part of the establishment of this portal, a number of bioinformatic tools will need to be employed to seamlessly migrate existing genome-scale datasets into a common interface.The broader impact of this proposal relates to the important impact this effort will have on the entire international CHO biotechnology community. Partners from a wide range of international entities including academic, government, and industrial scientists, have come together to support the CHO genome community by providing resources, expertise, and data. Additional impact will come from the education of graduate students working on the project who will closely interface with industrial scientists and from workforce development activities for the industrial community.

本EAGER提案的总体目标是建立一个框架，以支持国际中国仓鼠卵巢（CHO）细胞社区进入基因组。CHO细胞是目前应用最广泛的用于生物制药生产的哺乳动物细胞系。在所有重组生物治疗药物中，近70%是在CHO细胞中生产的，每年的全球市场规模接近1000亿美元。生物制药生产将在未来几年继续为制药业提供巨大的增长，使新的医疗方法的持续研究和生产设施的扩大成为可能。不幸的是，由于缺乏基因组和社区基因组框架，使用代谢工程和其他科学和工程方法改进系统的努力已经滞后。因此，该项目将实施基础设施，以实现支持国际CHO社区的两个具体目标：具体目标#1：开发信息技术（IT）基础设施，以支持CHO生物技术社区共享基因组规模数据和工具，并提供CHO生物技术社区可以在线互动和协同的论坛。具体目标#2：教育研究生使用生物信息学来实施和分析CHO基因组，并使用对CHO社区重要的新的基于网络的工具提高特定的生产力和产品质量。这个建议的智力价值在于开发一个一站式的门户网站来支持社区。作为建立该门户的一部分，将需要使用许多生物信息学工具来无缝地将现有的基因组规模数据集迁移到一个公共接口。这项建议的更广泛影响涉及这项努力将对整个国际CHO生物技术界产生的重要影响。来自学术、政府和工业科学家等广泛国际实体的合作伙伴聚集在一起，通过提供资源、专业知识和数据来支持CHO基因组社区。另外的影响将来自于对从事该项目的研究生的教育，他们将与工业科学家密切接触，并来自于工业社区的劳动力发展活动。