Lipidology of host cells and viral envelopes

宿主细胞和病毒包膜的脂质学

• 批准号: 13166150
• 负责人: Professorin Dr. Britta Brügger
• 金额: --
• 依托单位: Biochemie-Zentrum (BZH)
• 依托单位国家: 德国
• 项目类别: Priority Programmes
• 财政年份: 2005
• 资助国家: 德国
• 起止时间: 2004-12-31 至 2012-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/13166150?language=en
• 关键词: Lipidology; host; cells; viral; envelopes

We have developed a method by nano-ESI-MS/MS to qualitatively and quantitatively assess membrane lipids including each of their molecular species at the low picomole level. This method has opened the door to compare lipid compositions of membrane systems that, at a highly purified stage, are available only in minute quantities. This novel and hitherto not widely available method will be used to quantitatively analyse the lipid compositions of viral particles in order to compare them with their host membranes. These investigations are instrumental for our understanding of the molecular mechanisms that underlie formation of virus particles.

我们开发了一种纳米esi -MS/MS方法，用于在低皮摩尔水平上定性和定量地评估膜脂，包括它们的每个分子种类。这种方法为比较膜系统的脂质组成打开了大门，在高度纯化阶段，只有少量可用。这种新颖且迄今尚未广泛使用的方法将用于定量分析病毒颗粒的脂质组成，以便将它们与宿主膜进行比较。这些研究有助于我们了解病毒颗粒形成的分子机制。