Lipidology of host cells and viral envelopes

我们开发了一种通过nano-ESI-MS/MS定性和定量评估膜脂质的方法，包括在低皮摩尔水平下的每种分子种类。这种方法打开了门比较脂质组成的膜系统，在高度纯化的阶段，只有在微量。这种新的和迄今尚未广泛使用的方法将用于定量分析病毒颗粒的脂质成分，以比较它们与它们的宿主膜。这些研究有助于我们理解病毒颗粒形成的分子机制。

We have developed a method by nano-ESI-MS/MS to qualitatively and quantitatively assess membrane lipids including each of their molecular species at the low picomole level. This method has opened the door to compare lipid compositions of membrane systems that, at a highly purified stage, are available only in minute quantities. This novel and hitherto not widely available method will be used to quantitatively analyse the lipid compositions of viral particles in order to compare them with their host membranes. These investigations are instrumental for our understanding of the molecular mechanisms that underlie formation of virus particles.