The mechanism of molybdenum cofactor-insertion into apo-enzymes of the sulfite oxidase-class

钼辅因子插入亚硫酸氧化酶类脱辅基酶的机制

• 批准号: 131890350
• 负责人: Professor Dr. Ralf R. Mendel
• 金额: --
• 依托单位: Institut für Pflanzenbiologie
• 依托单位国家: 德国
• 项目类别: Research Units
• 财政年份: 2009
• 资助国家: 德国
• 起止时间: 2008-12-31 至 2015-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/131890350?language=en
• 关键词: mechanism; molybdenum; cofactor; insertion; into

In the previous part of the project we had focused on the molybdenum cofactor (Moco)- binding proteins mediating transfer of Moco between the site of its synthesis and the final user enzymes. Now in the second project phase, we will focus in detail on the mechanism of Moco insertion itself. For a better understanding of Moco insertion into target enzymes it is essential to have well characterized systems available both for the Moco donor and for the Moco-free acceptor. There are a number of broad objectives that we wish to achieve: (1) Unveil the mechanism by which Moco is formed on the Moco donor protein Cnx1 (Arabidopsis) and Nit-9 (Neurospora). (2) Characterize the Moco-free form of the acceptor protein sulfite oxidase from Neurospora. In contrast to plants, Neurospora possesses the animal-like two-domain form of sulfite oxidase consisting of a Moco-domain and a hemedomain. (3) Unveil the mechanism by which Moco is inserted into apo-sulfite oxidase. Like in humans and other mammals, mature and functional Neurospora sulfite oxidase is localized in the intermembrane space of mitochondria. At what stage and where (cytosol? intermembrane space?) Moco becomes inserted? Which machinery provides Moco?

在该项目的前一部分，我们集中在钼辅因子（Moco）结合蛋白介导的Moco之间的网站的合成和最终用户酶的转移。现在在项目的第二阶段，我们将详细关注Moco插入本身的机制。为了更好地理解Moco插入到靶酶中，必须具有可用于Moco供体和无Moco受体的良好表征的系统。我们希望实现一些广泛的目标：（1）揭示Moco在Moco供体蛋白Cnx 1（拟南芥）和Nit-9（脉孢菌）上形成的机制。(2)表征脉孢菌受体蛋白亚硫酸盐氧化酶的无Moco形式。与植物相比，脉孢菌具有类似动物的双结构域形式的亚硫酸盐氧化酶，其由Moco结构域和血红素域组成。(3)揭示Moco插入脱亚硫酸盐氧化酶的机制。与人类和其他哺乳动物一样，成熟和功能性的脉孢菌亚硫酸盐氧化酶定位于线粒体的膜间隙中。在什么阶段和在哪里（细胞质？膜间隙？）莫科被插入？哪些机器提供Moco？

项目成果

The biosynthesis of the molybdenum cofactors

• DOI: 10.1007/s00775-014-1173-y
• 发表时间: 2015-03-01
• 期刊: JOURNAL OF BIOLOGICAL INORGANIC CHEMISTRY
• 影响因子: 3
• 作者: Mendel, Ralf R.;Leimkuehler, Silke
• 通讯作者: Leimkuehler, Silke