Dissertation Research: Experimental in Vivo Evolution of the RNase P RNA Ribozyme

论文研究：RNase P RNA 核酶的体内进化实验

• 批准号: 9902351
• 负责人: Robert Dorit
• 金额: $ 1万
• 依托单位: Yale University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-06-15 至 2001-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9902351&HistoricalAwards=false
• 关键词: Dissertation; Research; Experimental; Vivo; Evolution

Dorit9902351This study uses an experimental approach to explore a fundamental component of all metabolisms, the ribonucleoprotein RNase P. Composed of a catalytically active RNA and a companion protein, RNase P is present in all organisms, albeit in different forms. The investigators' objective is to understand the forces that have shaped the diversity of RNase P. To do so, they have developed a system for in vivo evolution that allows them to introduce an RNase P from a different organism into the E.coli bacterium. They will then be in a position to follow the evolution of this introduced RNase P in its new cellular environment, providing a direct glimpse into the evolution of single component of complex metabolisms. This approach will not only shed light on the action and flexibility of RNase P, but is in effect a generalizable experimental strategy for investigating the function of any protein or catalytic RNA of interest. This approach also extends the power of evolutionary analysis by providing a dynamic, experimentally based complement to more conventional retrospective approaches.

Dorit9902351这项研究使用实验方法来探索所有代谢的基本组成部分，核糖核蛋白RNase P。由催化活性RNA和伴侣蛋白组成，RNase P存在于所有生物体中，尽管形式不同。 研究人员的目标是了解形成RNase P多样性的力量，为此，他们开发了一种体内进化系统，使他们能够将来自不同生物体的RNase P引入大肠杆菌细菌。 然后，他们将能够跟踪这种引入的RNase P在其新的细胞环境中的进化，从而直接了解复杂代谢的单一组分的进化。 这种方法不仅揭示了RNase P的作用和灵活性，而且实际上是研究任何感兴趣的蛋白质或催化RNA功能的可推广的实验策略。 这种方法也扩展了进化分析的力量，提供了一个动态的，基于实验的补充，更传统的回顾性方法。