Enzymatic Hydrolysis of Macromolecules: Development of Complex Substrates and Investigation of Hydrolysis Rates in Seawater and Sediments

大分子的酶水解：复杂底物的开发以及海水和沉积物中水解速率的研究

• 批准号: 9906089
• 负责人: Carol Arnosti
• 金额: $ 28万
• 依托单位: University of North Carolina at Chapel Hill
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-09-01 至 2003-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9906089&HistoricalAwards=false
• 关键词: Enzymatic; Hydrolysis; Macromolecules; Development; Complex

ABSTRACTOCE-9906089Most organic matter is biologically synthesized in the form of high molecular-weight macromolecules such as proteins, lipid complexes, and polysaccharides, the vast majority eventually being remineralized to carbon dioxide. Very little is known about the enzyme systems that mediate these important decomposition processes. For this reason, the principal investigator proposes to develop a set of substrates that can be used to investigate the rate of hydrolysis and substrate specificities of extracellular enzymes involved in the remineralization of polysaccharides and carbohydrate-containing macromolecules in the marine water column and sediments. It is expected that the macromolecular substrates developed in this project will expand our current ability to examine extracellular enzymatic hydrolysis processes as well as our knowledge of the structure of naturally-occurring organic macromolecules.

Abstractoce-9906089最有机物是生物学合成的，以高分子量的大分子分子（例如蛋白质，脂质复合物和多糖）的形式，最终被回想为二氧化碳。 关于介导这些重要分解过程的酶系统知之甚少。 因此，首席研究者建议开发一组底物，可用于研究参与多糖和碳水化合物含有碳水化合物的大量含量的细胞外酶的水解和底物特异性。 预计该项目中开发的大分子底物将扩大我们目前检查细胞外酶水解过程的能力，以及我们对天然有机大分子的结构的了解。