Fluorescence-Based Diagnostic of Iron Limitation for Analysis of Natural Phytoplankton Communities

基于荧光的铁限制诊断，用于天然浮游植物群落分析

• 批准号: 9906635
• 负责人: Michael Behrenfeld
• 金额: $ 29.66万
• 依托单位: Rutgers University New Brunswick
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-12-01 至 2003-11-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9906635&HistoricalAwards=false
• 关键词: Fluorescence; Based; Diagnostic; Iron; Limitation

The overall objective of this project is to investigate iron limitation in marine phytoplankton using the newly discovered fluorescence diagnostic technique. The project encompasses both laboratory and field components. The laboratory studies are aimed at resolving the physiological mechanism(s) for the fluorescence diagnostic of iron limitation and testing for its expression across major phytoplankton taxonomic divisions. These studies are based on continuous culture experiments and will include measurements of. (1) variable fluorescence parameters, (2) fluorescence emission spectra, (3) picosecond fluorescence lifetimes, and (4) the stoichiometric composition of the photosystems. The laboratory studies will address the following questions:(1) Does the fluorescence diagnostic result from an effect of iron limitation on the fluorescence signature of state transitions or the back-transfer of electrons from a highly reduced plastoquinone pool to the primary acceptor on Photosystem II?(2) Is the fluorescence diagnostic equally expressed in all taxonomic divisions under iron limiting conditions or is it restricted to prokaryotic photoautotrophs?The modest field component will be conducted to explore the utility of this technique in nature.

该项目的总体目标是利用新发现的荧光诊断技术研究海洋浮游植物中的铁限制。该项目包括实验室和现场部分。实验室研究旨在解决铁限制荧光诊断的生理机制，并测试其在主要浮游植物分类部门中的表达。这些研究基于连续培养实验，并将包括测量。 （1）可变荧光参数，（2）荧光发射光谱，（3）皮秒荧光寿命，（4）光系统的化学计量组成。实验室研究将解决以下问题：（1）荧光诊断是否是由于铁限制对状态转换的荧光特征或电子从高度还原的质体醌库到光系统II上的主要受体的反向转移的影响而产生的？（2）荧光诊断是否在铁限制条件下在所有分类学中均等表达，还是仅限于原核生物 光合自养生物？将进行适度的现场组件以探索该技术在自然界中的效用。