DNA Microarrays for Microbial Bioprocessing

本计画的目标是发展生物制程中细菌的DNA微阵列技术。 这项技术最近被开发出来，以加快识别特定功能微生物基因的过程。 该过程涉及在变化的环境条件下对转录产物mRNA的差异检测。 该方法已成功应用于真核生物，但不适用于mRNA不太稳定、浓度较低、特别是不具有多聚腺苷酸尾以便于分离的细菌。 E.大肠杆菌将被用作生物体的模型。 特别是，这项研究将优化微生物系统的RNA纯化，标记和杂交技术。 这些技术可能导致DNA微阵列在代谢工程和其他微生物问题的未来应用。

The goal of this project is to develop the DNA microarray technology for bacteria in bioprocessing. This technology has recently been developed to speed up the process of identifying microbial genes of specific function. The process involves the differential detection of the transcription product, mRNA, under changing environmental conditions. The method has been successfully applied to eucaryotes, but not to bacteria where the mRNA is less stable, is in lower concentrations, and, in particular, does not have a poly-A tail to facilitate isolation. E. coli will be used as a model for organisms. In particular, this research will optimize RNA purification, labeling, and hybridization techniques for microbial systems. These technologies could lead to future applications of the DNA microarray to metabolic engineering and other microbial problems.