Metabolomics: Development of novel metabolic analysis system for 1-butanol production

代谢组学：开发用于 1-丁醇生产的新型代谢分析系统

• 批准号: 1139318
• 负责人: James Liao
• 金额: $ 149.69万
• 依托单位: University of California-Los Angeles
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-12-01 至 2017-11-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1139318&HistoricalAwards=false
• 关键词: Metabolomics; Development; novel; metabolic; analysis

Biological engineering offers great promise for the production of next generation biofuels that are a sustainable alternative to fossil fuels. This research project will use novel high-throughput technologies to understand how microorganisms, such as baker's yeast and photosynthetic bacteria, produce these fuel molecules as part of their natural metabolism. Just as advances in DNA sequencing technology have made it possible to read whole genomes, new methods allow scientists to measure the metabolome, all the chemicals produced by the cell's metabolism. This project will combine a deep understanding of the genes and the chemical processes inside the cell and use novel computer modeling approaches to guide genetic engineering approaches that will boost the production of butanol, an advanced biofuel. Broader Impacts This project will integrate advanced training opportunities for young scientists in both the US and Japan through collaboration between the University of California, Los Angeles, Osaka University, Kobe University, and the Kaiteki Institute of the Mitsubishi Chemical Company in Japan. The training of students and postdoctoral fellows will be enriched through an exchange program between the participating laboratories, and by participation in an international workshop on the new technologies being developed in this project. A goal of this project is to train the next generation of researchers in the field of environmentally-conscious biofuels.

生物工程为下一代生物燃料的生产提供了巨大的希望，这种燃料是化石燃料的可持续替代品。这项研究项目将使用新的高通量技术来了解微生物，如面包酵母和光合细菌，如何产生这些燃料分子，作为其自然新陈代谢的一部分。就像DNA测序技术的进步使阅读整个基因组成为可能一样，新的方法使科学家能够测量代谢组，即细胞新陈代谢产生的所有化学物质。该项目将结合对基因和细胞内化学过程的深入了解，并使用新的计算机建模方法来指导基因工程方法，以促进先进生物燃料丁醇的生产。更广泛的影响该项目将通过加州大学洛杉矶分校、大阪大学、神户大学和日本三菱化学公司的Kaiteki研究所的合作，整合美国和日本年轻科学家的高级培训机会。通过参与实验室之间的交流计划以及参加关于该项目正在开发的新技术的国际研讨会，将丰富对学生和博士后研究员的培训。该项目的一个目标是培训环保生物燃料领域的下一代研究人员。