Microbiological and Biochemical Analysis of Phosphorus Accumulating Organisms in Full Scale Wastewater Treatment Plants

9909103 Noguera在该奖项的支持下进行的研究的目的是调查在全面的污水处理厂中去除磷酸盐的微生物学和生物化学，作为改善生物处理工艺操作的步骤，以提高从废水中去除磷酸盐的能力。 实验室工作涉及应用一种基于积累磷酸盐的微生物细胞与不积累磷酸盐的微生物细胞之间浮力密度差异的技术，作为分离和隔离磷酸盐的物理方法。 在这项工作中，一个程序将用于可视化的多磷酸盐染色结合荧光杂交，以允许同时识别微生物和测定其积累的多磷酸盐。 实验室规模的研究结果将与从位于威斯康星州的麦迪逊、斯托顿和拉辛的废水处理厂的微生物聚磷群落的研究中获得的知识相关联。 麦迪逊的废水处理厂使用改进的UCT（开普敦大学）生物处理工艺，斯托顿厂操作高级氧化工艺，而拉辛废水处理厂使用常规的悬浮微生物生长、非增强生物磷酸盐去除工艺。 这项研究的结果预计将获得相关的知识，以改善工程设计和操作的处理厂和系统，利用微生物的代谢活动，从废水中去除植物营养物质。 这项工作是补充研究正在进行的支持下，由美国国家科学基金会批准号9875642，其目的是调查的微生物生态学的氮去除废水曝气缺氧过程。 ***

9909103 Noguera The objective of research being conducted with support by this award is to investigate the microbiology and biochemistry of phosphate removal in full-scale wastewater treatment plants as steps toward improvement in operation of biotreatment processes for enhanced removal of phosphates from wastewater. Laboratory work involves application of a technique based on differences of buoyant densities between cells of microorganisms that accumulate phosphates from those that do not accumulate them as a physical method for their separation and isolation. In this work, a procedure will be used for visualization of polyphosphate staining in combination with fluorescent hybridization to allow simultaneous identification of microorganisms and determination of their accumulations of polyphosphates. Results of laboratory-scale research will be correlated with knowledge obtained from studies of the microbial phosphorus accumulating communities of wastewater treatment plants located in Madison, Stoughton and Racine, Wisconsin. the wastewater treatment plant in Madison uses a modified UCT (University of Cape Town) biotreatment process, the Stoughton plant operates an advanced oxidation process and the Racine wastewater treatment plant uses a conventional suspended microbial growth, non-enhanced biological phosphate removal process. Results of this research are expect to relate knowledge obtained to improvement in the engineering design and operation of treatment plants and systems that utilize metabolic activities of microorganisms to remove plant nutrients from wastewater. This work is complementary to research being conducted with support from NSF Grant No. 9875642, the objective of which is to investigate the microbial ecology of nitrogen removal from wastewater by aerated-anoxic processes. ***