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Engineering and Analysis of Pressure Sensitive Antibodies

Engineering and Analysis of Pressure Sensitive Antibodies
压敏抗体的工程与分析
批准号:
9910186
负责人:
Martin Yarmush
金额:
$41.66万
依托单位国家:
美国
项目类别:
Continuing Grant
财政年份:
2000
资助国家:
美国
项目状态:
已结题
起止时间:
2000-09-15 至 2003-08-31

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中文摘要
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英文摘要
The objective of this project is to exploit the pressure sensitivity of antigen-antibody complexes for applications in biotechnology. The specific goals of this research are: (1) to investigate in detail the effect of salt on several different antigen-antibody complexes to provide guidelines and strategies for modulating the pressure effect; (2) to investigate changes in the pressure sensitivity of a purely hydrophobic antigen-antibody complex, digoxigenin:26-10, using a number of mutants of the antibody as well as environmental perturbations; and (3) to determine suitable operating conditions for performing pressure elution separations.
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