ME: A Functional Proteomics Approach for Elucidation of Metabolic Pathways: Aromatic Biosynthesis in Archaeoglobus Fulgidus

本项目的目的是建立一个功能蛋白质组学的方法，阐明的重要芳香族生物合成途径的极端thermoplilic硫酸盐还原古菌，古生球菌fulgidus，其基因组最近被测序。 特别是，主要研究者将设计基于液相色谱/质谱（LC/MS）、DNA微阵列技术和E.大肠杆菌中进行酶活性筛选和基因鉴定。

The objective of this project is to establish a functional proteomics approach for the elucidation of the important aromatic biosynthesis pathways of the extremely thermoplilic sulfate-reducing archaeon, Archaeoglobus fulgidus, whose genome was recently sequenced. In particular, the Principal Investigators will design techniques based on the coordinated use of liquid chromatography/mass spectrometry (LC/MS), DNA microarray technologies, and gene cloning and expression in E. coli for efficient screening of enzyme activities and for gene identification.