ME: A Functional Proteomics Approach for Elucidation of Metabolic Pathways: Aromatic Biosynthesis in Archaeoglobus Fulgidus

ME：阐明代谢途径的功能蛋白质组学方法：Archaeoglobus Fulgidus 中的芳香生物合成

• 批准号: 9911718
• 负责人: Harold Monbouquette
• 金额: $ 45万
• 依托单位: University of California-Los Angeles
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-06-01 至 2003-11-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9911718&HistoricalAwards=false
• 关键词: ME; Functional; Proteomics; Approach; Elucidation

The objective of this project is to establish a functional proteomics approach for the elucidation of the important aromatic biosynthesis pathways of the extremely thermoplilic sulfate-reducing archaeon, Archaeoglobus fulgidus, whose genome was recently sequenced. In particular, the Principal Investigators will design techniques based on the coordinated use of liquid chromatography/mass spectrometry (LC/MS), DNA microarray technologies, and gene cloning and expression in E. coli for efficient screening of enzyme activities and for gene identification.

本项目的目的是建立一个功能蛋白质组学的方法，阐明的重要芳香族生物合成途径的极端thermoplilic硫酸盐还原古菌，古生球菌fulgidus，其基因组最近被测序。 特别是，主要研究者将设计基于液相色谱/质谱（LC/MS）、DNA微阵列技术和E.大肠杆菌中进行酶活性筛选和基因鉴定。