Synthesis of Oxygenated Hydrocarbons by Cytochrome P-450 Electroenzymology

细胞色素 P-450 电酶法合成含氧烃

• 批准号: 9313009
• 负责人: Harold Monbouquette
• 金额: $ 26万
• 依托单位: University of California-Los Angeles
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-08-01 至 1997-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9313009&HistoricalAwards=false
• 关键词: Synthesis; Oxygenated; Hydrocarbons; Cytochrome; 450

Vincent Vilker 9313009 This work is directed toward development of an economical synthesis of oxygenated hydrocarbons using active cytochrome P-450 immobilized on a working electrode. The immediate objectives are to determine stoichiometries, product distributions, and kinetics of the hydroxylation of hydrocarbons by NADH-driven P-450, to develop modified electrodes for the regeneration of P-450, and to compare products, rates, and yields of the NADH and electrochemically regenerated cytochrome P-450. Two forms of cytochrome are studied: P-450cam and P-450soy; the similarities and differences are examined. Hydrocarbon substrates include norcamphor, -pinene, 1,2,3,4-tetrahydronaphthalene (tetralin), decahydronaphthalene (decalin), and naphthalene. Regiospecificity and stereospecificity are examined, turnover rates on the cytochrome are measured, and models of the process are developed. This should lead to an energy-efficient bioelectrochemical process that combines the efficiency of electrocatalysts with the specificity of enzymes, thus producing good yields of high-value products with little unwanted side reaction. The project is done in collaboration with the duPont Company.

文森特·维尔克9313009 这项工作是针对开发一种经济的合成含氧烃使用活性细胞色素P-450固定在工作电极。 直接的目标是确定化学计量，产品分布，和动力学的碳氢化合物的羟基化由NADH驱动的P-450，开发用于再生的P-450的修饰电极，并比较产品，速率和产量的NADH和电化学再生的细胞色素P-450。 研究了两种形式的细胞色素：P-450 cam和P-450 soy;检查了相似性和差异。 烃类底物包括降樟脑、β-蒎烯、1，2，3，4-四氢萘（四氢化萘）、十氢萘（十氢化萘）和萘. 区域特异性和立体特异性进行检查，周转率的细胞色素进行测量，并开发模型的过程。 这将导致一种高能效的生物电化学过程，该过程将电催化剂的效率与酶的特异性相结合，从而产生高价值产品的良好产量，而几乎没有不必要的副反应。 该项目是与杜邦公司合作完成的。