U.S.-Japan Cooperative Science: Characterization of Alkaloid Biosynthetic Genes in Nicotiana Tabacum

美日合作科学：烟草生物碱生物合成基因的表征

• 批准号: 9912099
• 负责人: John Jelesko
• 金额: $ 2.65万
• 依托单位: Virginia Polytechnic Institute and State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-07-15 至 2002-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9912099&HistoricalAwards=false
• 关键词: U.S.; Japan; Cooperative; Science; Characterization

9912099JeleskoThis award supports a two-year collaborative research project between Professor John Jelesko of Virginia Polytechnic Institute in Blacksburg, Virginia and Dr. Masaki Furuya of the Hitachi Advanced Research Laboratory in Saitama, Japan. The researchers will be undertaking a study of the characterization of alkaloid biosynthetic genes in Nicotiana Tabacum. Tropane alkaloids are important secondary plant metabolites involved in plant chemical ecology. Despite the importance of plant alkaloids little is known about the molecular biology of alkaloid biosynthetic genes or their regulation. The goal of this research project is to use an advanced transcription profiling method, called Fluorescent Differential Display (FDD), to identify and isolate tropane alkaloid biosynthetic genes in Nicotiana Tabacum (tobacco). The researchers will perform several large-scale FDD screens to identify alkaloid biosynthetic genes in tobacco. They will attempt to identify genes that are differentially expressed between a wild type tobacco accession and mutant tobacco accessions that have defined double and single mutations that greatly reduce tropane alkaloid levels. By utilizing specific root culture conditions and defined mutations in near isogenic backgrounds they will identify differentially expressed genes specifically involved in alkaloid biosynthesis. This approach overcomes the difficulties inherent with traditional biochemical and genetic methods in Nicotiana species. The project brings together the efforts of two laboratories that have complementary expertise and research capabilities. The research should provide important information on alkaloid biosynthesis in plants, which should then result in potential applications in plant biotechnology. The research will also be of interest to the pharmaceutical industries. The project advances international human resources through the participation of a postdoc and a graduate student. Through the exchange of ideas and technology, this project will broaden our base of basic knowledge and promote international understanding and cooperation. Results of the research will be published in international scientific journals and will also be presented at scientific meetings in the U.S. and abroad.

9912099 Jelesko该奖项支持弗吉尼亚州布莱克斯堡弗吉尼亚理工学院的John Jelesko教授和日本崎玉日立高级研究实验室的Masaki Furuya博士之间为期两年的合作研究项目。研究人员将对烟草中生物碱生物合成基因的特征进行研究。托烷生物碱是植物化学生态中重要的次生代谢产物。尽管植物生物碱很重要，但人们对生物碱生物合成基因的分子生物学或其调控知之甚少。本研究项目的目的是使用一种先进的转录图谱方法，称为荧光差异显示(FDD)，来鉴定和分离烟草中的托烷生物碱生物合成基因。研究人员将进行几次大规模的FDD筛查，以识别烟草中的生物碱生物合成基因。他们将试图识别野生型烟草品种和突变烟草品种之间差异表达的基因，突变烟草品种定义了双突变和单突变，这些突变大大降低了托烷生物碱的水平。通过利用特定的根培养条件和在近等基因背景下定义的突变，他们将识别特定涉及生物碱生物合成的差异表达基因。这种方法克服了传统的生化和遗传方法在烟草物种中所固有的困难。该项目汇集了两个拥有互补专业知识和研究能力的实验室的努力。这项研究应该提供有关植物生物碱生物合成的重要信息，从而在植物生物技术中产生潜在的应用。这项研究也将引起制药行业的兴趣。该项目通过博士后和研究生的参与促进了国际人力资源的发展。通过思想和技术的交流，这个项目将扩大我们的基础知识基础，促进国际理解与合作。研究结果将发表在国际科学期刊上，并将在美国和国外的科学会议上公布。