Determining the necessity and mechanisms of assortative mating in an adaptively diverging salamander population (Salamandra salamandra)

确定适应性分化蝾螈种群（Salamandra salamandra）中选型交配的必要性和机制

• 批准号: 135720878
• 负责人: Professorin Dr. Barbara Caspers
• 金额: --
• 依托单位: Arbeitsgruppe Verhaltensforschung
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2009
• 资助国家: 德国
• 起止时间: 2008-12-31 至 2013-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/135720878?language=en
• 关键词: Determining; necessity; mechanisms; assortative; mating

Assortative mating – preferred mating with the own type - is a central theme within the concept of adaptive speciation, as it is required to prevent geneflow between differentially adapted types. Recent studies on a population of the terrestrial fire salamander (Salamandra salamandra) in Germany (the Kottenforst near Bonn) suggest that the adaptation to different reproduction habitats within the small forest initiated a process of adaptive divergence under sympatric conditions. This proposal is aimed to analyse the mechanisms and necessity of assortative mating in this system. We will investigate the degree of assortative mating under seminatural condition, in order to find existing isolation mechanisms. As odour cues are widely used by salamanders for intraspecific communication and sexual recognition, we will analyse in behavioural/genetic based mate-choice experiments whether odour cues are involved in habitat dependent assortative mating. Further, by applying a bioassay approach in combination with high performance liquid chromatography linked with mass spectrometry (HPLC/MS) we are going to analyse and identify involved odour substances chemically. In order to correlate and interpret the results from the assortative mating analysis in a natural context we will determine the degree of sympatry of adaptive types by analysing dispersal, home range size and movement patterns of adult individuals across the Kottenforst over several years. This detailed study is an outstanding approach to analyse mechanisms of assortative mating in a natural setting of an adaptively diverging terrestrial vertebrate species.

选择性交配（Assortative mating）--偏好与自己的类型交配--是适应性物种形成概念中的一个中心主题，因为它需要防止不同适应类型之间的基因流动。最近对德国（波恩附近的Kottenforst）的陆生火蝾螈（Salamandra salamandra）种群的研究表明，在同域条件下，对小森林内不同繁殖生境的适应启动了一个适应性分化的过程。本研究旨在分析该系统中选择性交配的机理和必要性。我们将在自然条件下研究排斥交配的程度，以发现现有的隔离机制。由于气味线索被广泛使用的蝾螈种内通信和性识别，我们将分析在行为/遗传为基础的择偶实验是否气味线索参与栖息地依赖性交配。此外，通过将生物测定方法与高效液相色谱-质谱联用（HPLC/MS）相结合，我们将以化学方式分析和鉴定所涉及的气味物质。为了关联和解释的结果，在一个自然的情况下，我们将确定共域的自适应类型的传播，家庭范围的大小和运动模式的成年个体在科滕福斯特几年的分析。这项详细的研究是一个杰出的方法来分析机制的适应性分歧陆生脊椎动物物种在自然环境中的交配。