Instrumentation for Instruction in Analytical Separations

化学(12)虽然分离科学在化学研究的许多领域都很重要，但它是一个经常被忽视或没有向本科生观众充分展示的领域。实验工作往往没有充分利用现代高效液相色谱仪的特点。在本科阶段，接触毛细管电泳仪(CE)是极其有限的。通常，这些乐器都是预留给高级别课程使用的。这个项目正在解决典型本科教育项目在分居经验方面的缺陷。在下级和上级都引入了使用高效液相色谱和毛细管电泳仪的实验室。学生们反复遇到这两种工具，这些工具的独特功能和局限性被突显出来。该项目从教育文献和研究文献中进行实验，并对这些实验进行修改，以便学生在改变实验参数时调查影响。随着学生从低年级升到高年级，他们的经验积累了，他们在实验中的决策作用也增加了。这是可能的，因为通过反复接触这些仪器，学生能够探索每种分离技术的不同操作模式。在初级和高级阶段，他们选择最适合其特定和个性化样本的方法。鼓励学生与其他学生团体合作。预期的总体结果是，学生将对高效液相色谱分离和毛细管电泳色谱分离的能力和局限性有一个深入的了解。没有完成完整课程顺序的低年级学生正在获得对用于分析混合物的技术的欣赏。高年级学生能够智能地预测各种实验参数对给定分离的影响。也许最重要的是，他们能够在特定分析的柱类型、流动相和衍生化方面选择最有可能成功的方法。评估计划包括一个组成部分，该组成部分将跟踪学生反复接触仪器对学生使用高效液相色谱和毛细管电泳法设计有效分离的能力产生的影响。

Chemistry (12) Although separation science is of fundamental importance in many areas of chemical investigations, it is an area which is often neglected or inadequately presented to undergraduate audiences. Experimental endeavors often do not take full advantage of the features of modern high-performance liquid chromatography (HPLC) instrumentation. Exposure to capillary electrophoresis instrumentation (CE) is extremely limited at the undergraduate level. Frequently, these pieces of instrumentation are reserved for use in the upper division courses. This project is addressing the shortcomings of typical undergraduate educational programs in terms of the experience with separations. Laboratories using HPLC and CE instrumentation are being introduced at both the lower and upper divisional levels. Students are repeatedly encountering both instruments, and the unique capabilities and limitations of these instruments are being highlighted. The project is taking experiments from the educational literature and from the research literature and is modifying these experiments so that students investigate the effects when they change experimental parameters. As students gain in experience as they move from the lower to upper division, their decision making role in the experiments increases. This is possible because with repeated exposure to the instruments, students are able to explore the different modes of operation of each separation technique. At the junior and senior level, they choose the methods most appropriate to their particular and individualized samples. Students are encouraged to collaborate with other student groups. The expected overall outcome is that students will have a sophisticated understanding of the capabilities and limitations of HPLC and CE separations. Lower division students who do not complete the full course sequence are gaining an appreciation for the techniques used to analyze mixtures. Upper division students are able to make intelligent predictions of the effects of various experimental parameters on a given separation. Perhaps most importantly, they are able to choose the most likely successful approach in terms of column type, mobile phase, and derivatization chemistry for a given analysis. The evaluation plan includes a component that will track the impact of this repeated exposure by students to the instrumentation on the ability of the students to develop increasing sophistication in designing effective separations using HPLC and CE.