SBIR Phase I: Disease Block: Genetically Engineered Plants with Disease Resistance

SBIR 第一阶段：疾病阻断：具有抗病性的基因工程植物

• 批准号: 9960981
• 负责人: Chandrika Ramadugu
• 金额: $ 10万
• 依托单位: Integrated Plant Genetics Inc.
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-01-01 至 2000-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9960981&HistoricalAwards=false
• 关键词: SBIR; Phase; Disease; Block; Genetically

This Small Business Innovation Research Phase I project is to perfect a method usingpeptide aptamers expressed in plants that appears to work to control certain bacterialplant pathogens in the genus Xanthomonas. The aptamers block function of criticalpathogenicity (Pth) proteins that are literally injected by the pathogens into plantcells. The objectives are to 1) select better aptamers for capacity to block diseasedevelopment by screening for binding to predicted critical regions on the Pth protein and2) create transgenic citrus plants that express the aptamers for later testing forresistance to citrus canker disease. Phase II funding will be sought to produce and testtransgenic rice and common bean plants genetically engineered to produce the aptamers.Three diseases of economic importance to the U.S. are likely to be controlled by thisnovel genetic mechanism: citrus canker, common bean blight and rice blight. There are nocures, no stable genetic resistance, nor even satisfactory controls for these diseases.The citrus industry and state regulators consider canker control methods to be a toppriority. Localized losses of citrus, bean and rice due to these three diseases can be upto 50%, 20% and 100%, respectively. Applications to other diseases are likely by this newgene engineering technology.

这个小企业创新研究第一阶段项目是完善一种使用植物中表达的肽适体的方法，该方法似乎可以控制黄单胞菌属中的某些细菌植物病原体。适体阻断病原体注入植物细胞的关键致病性（Pth）蛋白的功能。目的是1）通过筛选与预测的Pth蛋白关键区域的结合来选择更好的能够阻断疾病发展的适体; 2）创建表达适体的转基因柑橘植物，用于以后测试对柑橘溃疡病的抗性。第二阶段的资金将用于生产和测试转基因水稻和普通豆类植物，这些植物通过基因工程来产生适体。对美国具有重要经济意义的三种疾病可能通过这种新的遗传机制来控制：柑橘溃疡病、普通豆类枯萎病和水稻枯萎病。目前还没有治愈方法，没有稳定的遗传抗性，甚至也没有令人满意的控制这些疾病的方法。柑橘产业和国家监管机构认为溃疡病控制方法是当务之急。这三种病害对柑桔、豆类和水稻的局部损失分别可达50%、20%和100%。这种新的基因工程技术很可能应用于其他疾病。