SBIR Phase I: Expression Pattern Screening for Agriculture Genomics

SBIR 第一阶段：农业基因组表达模式筛选

• 批准号: 9961034
• 负责人: Richard Kris
• 金额: $ 10万
• 依托单位: Nuvogen Research
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-01-01 至 2000-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9961034&HistoricalAwards=false
• 关键词: SBIR; Phase; Expression; Pattern; Screening

This Small Business Innovation Research Phase I project proposes to develop a high throughput multiple mRNA assay for screening large numbers of compounds to discover potential compounds that affect patterns of gene expression in plant tissue. The expression for a group of genes of interest and control genes can be tested all at once within each well of a 96-well plate. Investigators can evaluate how 96 different compounds affect the expression pattern for these genes in each plate. This technology will address an unmet need of the agricultural industry - to make efficient use of novel genomics information. This will allow testing of more gene targets including newly identified genes, and will provide information about selectivity and specificity.Proprietary methods allow DNA array technology to be used for the high throughput multiple mRNA assay. Preliminary results demonstrate the feasibility of these methods using mammalian cells. DNA arrays can be applied within each well and retain hybridization specificity. The method is sensitive enough to detect genes expressed at one mRNA molecule per cell from cells grown within one well of a 96-well plate. The researchers propose to apply the approach to cultured plant cells, in order to form a reliable and sensitive screening technology for discovering new compounds that modulate gene expression in crops.

这个小企业创新研究第一阶段项目建议开发一种高通量多mRNA分析方法，用于筛选大量化合物，以发现影响植物组织中基因表达模式的潜在化合物。一组感兴趣的基因和控制基因的表达可以在96孔板的每孔中同时进行测试。研究人员可以评估96种不同的化合物如何影响这些基因在每个培养皿中的表达模式。这项技术将解决农业尚未满足的需求——有效利用新的基因组学信息。这将允许测试更多的基因靶标，包括新发现的基因，并将提供有关选择性和特异性的信息。专有方法允许DNA阵列技术用于高通量多重mRNA分析。初步结果证明了这些方法在哺乳动物细胞中的可行性。DNA阵列可应用于每个孔内并保留杂交特异性。该方法足够灵敏，可以从生长在96孔板的一个孔内的细胞中检测每个细胞中一个mRNA分子表达的基因。这组科学家提议将这种方法应用于培养的植物细胞，以便形成一种可靠而敏感的筛选技术，用于发现调节作物基因表达的新化合物。