SBIR Phase II: Development and Manufacture of High-Density Plate Washer

SBIR二期：高密度洗板机的研发与制造

• 批准号: 0450448
• 负责人: Richard Kris
• 金额: --
• 依托单位: Nuvogen Research
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-01-15 至 2007-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0450448&HistoricalAwards=false
• 关键词: SBIR; Phase; II; Development; Manufacture

This Small Business Innovation Research (SBIR) Phase II project aims to develop a plate washer capable of washing very high-density plates, such as 1536 well plates, for ELISA and high-throughput screening assays. Currently, there are automated plate washers for 96 and 384 well plates, but there are none available for plates with ultra-high density. This is because currently available washer technology, employing a nozzle system, cannot be made reliable enough to allow dispense and aspirate nozzles to properly reach within each of the many, very small wells, and because the thin nozzles needed can get easily clogged using many standard buffers. In contrast, the proposed system uses a steady stream or sheet of solution, making the system less likely to clog. The commercial application of this project will be to allow use of high-throughput screening assays by industrial and academic researchers involved in genomics and drug discovery research. The proposed technology will enable additional use of fluorescent chemical compounds, that typically require a wash step to remove interfering substances, for screening.

该小型企业创新研究 (SBIR) 第二阶段项目旨在开发一种洗板机，能够清洗非常高密度的板，例如 1536 孔板，用于 ELISA 和高通量筛选测定。目前，有用于 96 和 384 孔板的自动洗板机，但没有适用于超高密度板的自动洗板机。 这是因为当前可用的采用喷嘴系统的清洗技术不能足够可靠以允许分配和抽吸喷嘴正确地到达许多非常小的孔中的每一个内，并且因为使用许多标准缓冲器时所需的薄喷嘴很容易被堵塞。相比之下，所提出的系统使用稳定的溶液流或片状溶液，使得系统不太可能堵塞。该项目的商业应用将允许参与基因组学和药物发现研究的工业和学术研究人员使用高通量筛选分析。拟议的技术将能够额外使用荧光化合物进行筛选，这些化合物通常需要清洗步骤来去除干扰物质。