SBIR Phase II: Expression Pattern Screening for Agriculture Genomics

SBIR 第二阶段：农业基因组表达模式筛选

• 批准号: 0110472
• 负责人: Richard Kris
• 金额: $ 50万
• 依托单位: Nuvogen Research
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-08-01 至 2003-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0110472&HistoricalAwards=false
• 关键词: SBIR; Phase; II; Expression; Pattern

This Small Business Innovation Research (SBIR) Phase II project will use a novel high throughput platform comprising of many, small gene arrays, contained within the wells of microtiter plates. This platform, termed Multi-Array Plate Screening (MAPS), allows simultaneous testing of the expression of a specific group of genes of interest and appropriate controls using RNA derived from 96 separate samples, within each well of a 96-well plate. MAPS provides the endpoint assay for a high throughput screen, in which investigators can evaluate how different chemical compounds, applied to cells, tissues, or organisms in vivo, affect the expression pattern for the genes of interest.This technology will address an unmet need of the agricultural industry to make efficient use of novel genomics information in a manner that does not require distribution of genetically modified organisms (GMOs) in the form of transgenic plants. The plants are grown in each well of a 96 well plate to facilitate high-throughput screening. The platform allows facile and efficient testing of gene targets including newly identified genes, and also provides important information about selectivity and specificity. The commercial potential from this project is in the agricultural market.

这个小型企业创新研究(SBIR)第二阶段项目将使用一种新型的高通量平台，该平台由许多包含在微滴定板孔内的小基因阵列组成。这个名为多阵列平板筛选(MAP)的平台允许使用从96孔板中的每一孔中的96个单独样本中提取的RNA来同时测试一组特定的感兴趣基因的表达和适当的对照。MAP为高通量筛选提供了终点分析，研究人员可以评估应用于细胞、组织或生物体内的不同化合物如何影响感兴趣基因的表达模式。这项技术将解决农业行业尚未满足的需求，即以不需要以转基因植物的形式分发转基因生物(GMO)的方式有效利用新的基因组信息。这些植物生长在96孔板的每一孔中，以便于高通量筛选。该平台可以方便有效地测试基因靶标，包括新识别的基因，并提供有关选择性和特异性的重要信息。这个项目的商业潜力在于农业市场。