IDBR: Development of an x-ray flow cytometer for single cell metal analysis

IDBR：开发用于单细胞金属分析的 X 射线流式细胞仪

• 批准号: 0852802
• 负责人: James Penner-Hahn
• 金额: $ 70.63万
• 依托单位: Regents of the University of Michigan - Ann Arbor
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-01 至 2013-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0852802&HistoricalAwards=false
• 关键词: IDBR; Development; ray; flow; cytometer

Trace elements (those present at the parts-per-million level) play a critical role in virtually all biological processes; they are essential to life but can also be toxic if present at the wrong concentration. Consequently, the trace element levels within cells tend to be tightly regulated, with a complex set of machinery to control the "inorganic physiology" of cells, that is, the regulation, uptake, storage, and efflux of trace elements. Although there have been many studies of trace elements in biology, virtually all have relied on bulk analyses (e.g., how much metal is present on average in an ensemble of cells; and how does this change as a function of chemical and/or biological treatment?). The new instrument that will be developed will permit the direct determination of the concentrations of trace elements in intact individual cells. The instrument will allow, for the first time, facile determination of the inorganic composition of individual cells, thereby providing direct insight into the distribution of compositions within the population, and permitting studies aimed at correlating other cellular properties with chemical composition. By permitting determination of not only average concentrations but also the cell-to-cell variations in individual concentrations, and by allowing these data to be correlated with conventional measures of cell status, this will provide a transformative capability, opening the possibility of dissecting the cellular roles of both essential and toxic trace elements with an unprecedented level

微量元素（以百万分之一水平存在的元素）在几乎所有生物过程中发挥着关键作用;它们是生命所必需的，但如果存在于错误的浓度，也可能是有毒的。 因此，细胞内的微量元素水平往往受到严格的调节，具有一套复杂的机制来控制细胞的“无机生理学”，即微量元素的调节、摄取、储存和流出。 虽然在生物学中已经有许多关于微量元素的研究，但几乎所有的研究都依赖于批量分析（例如，平均有多少金属存在于细胞系综中;以及作为化学和/或生物处理的函数，这是如何变化的？将开发的新仪器将允许直接测定完整单个细胞中微量元素的浓度。 该仪器将允许，为第一次，个别细胞的无机组成的简易测定，从而提供直接洞察人口内的组合物的分布，并允许旨在与化学组成的其他细胞特性的研究。 通过允许不仅测定平均浓度，而且测定单个浓度的细胞与细胞之间的变化，并通过允许这些数据与细胞状态的常规测量相关联，这将提供一种变革性的能力，打开了以前所未有的水平剖析必需和有毒微量元素的细胞作用的可能性