POWRE: Reusable Selection Cassettes for Gene Integration or Disruption in Mammalian Systems

POWRE：可重复使用的选择盒，用于哺乳动物系统中的基因整合或破坏

• 批准号: 9973438
• 负责人: Nancy Da Silva
• 金额: $ 7.5万
• 依托单位: University of California-Irvine
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-07-15 至 2001-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9973438&HistoricalAwards=false
• 关键词: POWRE; Reusable; Selection; Cassettes; Gene

The goal of this proposal is to develop a gene integration cassette for mammalian cells which eliminates the resistance selection problem common in DNA engineering efforts. All of the PI's previous work has been in yeast, and this proposal represents a significant move into mammalian systems which are quite different. A reusable selection cassette will be used for the gene insertion after which it will be removed and used for subsequent integrations. The use of this technique will be evaluated in two cell lines, CHO cells and a human alveolar epithelial cell line.

本提案的目标是开发一种用于哺乳动物细胞的基因整合盒，以消除DNA工程努力中常见的抗性选择问题。PI之前的所有工作都是在酵母中进行的，而这一提议代表着在哺乳动物系统中迈出了重要的一步，而哺乳动物系统与酵母完全不同。一个可重复使用的选择盒将用于基因插入，之后它将被移除并用于随后的整合。该技术的使用将在两种细胞系，CHO细胞和人肺泡上皮细胞系中进行评估。