GOALI: Metabolic Engineering of Yeast for Polyketide Synthesis

聚酮类化合物是一类种类繁多的天然产物，具有重要的人类治疗意义。尽管多酮类化合物可以在多种微生物中产生，但它们通常在天然宿主体内以微量产生。为了优化这些化合物的生产和产生新的聚酮，异源宿主具有几个优势。酵母的特殊属性与酿酒酵母中真菌聚酮的高水平生产的成功相结合，表明了这种宿主对聚酮生物合成的特殊承诺。首席研究员（PI）将开发和评估在酿酒酵母中引入和表达活性聚酮合成酶的各种策略。

DaSilva0432307Polyketides are a diverse group of natural products with great significance as human therapeutics. Although they are made in a variety of microorganisms, polyketides often are produced in their natural hosts in minute quantities. To optimize production of these compounds and for the generation of novel polyketides, heterologous hosts hold several advantages. The specific attributes of yeast combined with success at the high-level production of a fungal polyketide in Saccharomyces cerevisiae point to the exceptional promise of this host for polyketide biosynthesis. The Principal Investigator (PI) will develop and evaluate various strategies for introducing and expressing active polyketide synthases in S. cerevisiae.