GOALI: Metabolic Engineering of Yeast for Polyketide Synthesis

目标：用于聚酮化合物合成的酵母代谢工程

• 批准号: 0432307
• 负责人: Nancy Da Silva
• 金额: $ 11.77万
• 依托单位: University of California-Irvine
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-09-01 至 2006-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0432307&HistoricalAwards=false
• 关键词: GOALI; Metabolic; Engineering; Yeast; Polyketide

DaSilva0432307Polyketides are a diverse group of natural products with great significance as human therapeutics. Although they are made in a variety of microorganisms, polyketides often are produced in their natural hosts in minute quantities. To optimize production of these compounds and for the generation of novel polyketides, heterologous hosts hold several advantages. The specific attributes of yeast combined with success at the high-level production of a fungal polyketide in Saccharomyces cerevisiae point to the exceptional promise of this host for polyketide biosynthesis. The Principal Investigator (PI) will develop and evaluate various strategies for introducing and expressing active polyketide synthases in S. cerevisiae.

达席尔瓦0432307多酮类化合物是一类天然产物，具有重要的人体治疗作用。虽然它们是在各种微生物中产生的，但聚酮往往是在它们的自然宿主中以微量产生的。为了优化这些化合物的生产和产生新的聚酮，异源宿主具有几个优势。酵母的特殊属性与在酿酒酵母中高水平生产真菌聚酮的成功相结合，表明了这种宿主在聚酮生物合成方面的特殊前景。首席调查员(PI)将开发和评估在酿酒酵母中引入和表达活性聚酮合成酶的各种策略。