Amplification of Integrated Cloned Genes in Saccharomyces cerevisiae

微生物中克隆基因的稳定对于重组细胞的生产过程是极其重要的。这一建议的目标包括确定可能的扩增范围和增强、发生扩增的时间尺度和频率、开发和研究细胞内蛋白质生产的模型系统、确定对宿主菌株的影响、稳定性研究和种群建模。酵母反转录转座子为获得大量整合克隆基因提供了一种独特的途径。这项研究的结果应该证明这种独特的系统在稳定整合多个克隆基因方面的潜力，这种方法在许多情况下应该优于更常用的质粒系统。

The stabilization of cloned genes in microorganisms is extremely important for productive recombinant cell processes. The goals of this proposal include determining the extent and enhancement of amplification possible, the time-scale and frequency on which it occurs, development and study of a model system for intracellular protein production, determining the effects on the host strain, stability studies, and population modeling. Yeast retrotransposons offer a unique approach for obtaining large numbers of integrated cloned genes. The results from the research should demonstrate the potential of this unique system for the stable integrations of multiple cloned genes, a method, which in many instances, should be superior to the more commonly utilized plasmid systems.